摘要
目的探讨TNFα基因-308G>A和LTA基因252A>G多态性对PD发病潜在影响。方法利用Pyrosequencing技术对114例我国汉族散发性帕金森病(Parkinson's disease,PD)患者和133例正常对照进行TNFα-308G>A和LTA252A>G突变位点分析。结果病例组TNFα-308突变型(G/A+A/A)和野生型(G/G)阳性率与对照组相等,差异无统计学意义(χ2=3.46,P=0.0627)。病例组LTA252突变型(A/G+G/G)阳性率稍高于对照组,差异无统计学意义(χ2=0.28,P=0.5999)。单倍体型分析表明,TNFα-308杂合突变型(G/A)患者LTA252位点均为突变型(A/G或G/G)。结论TNFα-308和LTA252位点可能不是我国散发性PD发病易感因素。
Objective To inquire into the relevance of tumor necrosis factor alpha gene 308G〉A and Lymphotoxin alpha gene 252A〉 G to Chinese sporadic Parkinson＇s disease （PD） susceptibility. Methods 114 cases of sporadic PD patients and 133 age-and sex-matched controls were investigated with pyrosequencing technique. Results Comparing the frequencies of wild type （G/G） and mutation types （G/A ＋ A/A） of TNFα-308 between the cases and the controls revealed a minimal bordering but nonsignificant difference （X^2 = 3.46, P = 0. 062 7）. Genotype frequency of LTA 252 （G/G） in the PD cases was lower than that of the controls （0. 105 3 vs. 0. 135 3）. However, there was no significant difference between the cases and the controls. Haplotype analysis showed that heterozygous （G/A） mutation of TNFα-308 was always associated with mutated LTA 252 A/G or G/G. Conclusions TNFα-308 G〉 A and LTA 252A 〉 G might not be potential susceptibility locus leading to Chinese sporadic Parkinson＇s disease.
出处
《中华疾病控制杂志》
CAS
2009年第1期33-38,共6页
Chinese Journal of Disease Control & Prevention
基金
国家自然科学基金(30470591)
安徽省自然科学基金(050430716)
关键词
帕金森病
肿瘤坏死因子α
淋巴毒素
多态性
单核苷酸
Parkinson disease
Tumor necrosis factor-alpha
Lymphotoxin
Polymorphism, single nucleotide
