摘要
为了探明大麻快速生物脱胶的机制,在实验室条件下,利用脱胶高效菌株Dm111对大麻韧皮进行快速脱胶试验,定期测定胶质去除率和发酵液的相关指标。结果表明:在脱胶前期和中期,胶质去除率、脱胶菌活菌量不断增加,后期趋于平缓;果胶酶和木聚糖酶酶活均是脱胶前期增加缓慢,中期迅速增加,后期下降,而纤维素酶酶活在脱胶过程中变化不大,且酶活很低;pH值呈"V"型变化;还原糖出现2个峰值,呈近似"M"型变化;发酵液的COD、蛋白质和残渣量与脱胶时间呈正相关;至脱胶完成时,残渣量占大麻韧皮的27%左右。
In order to prove up the mechanism of hemp fast microbial retting, by using hemp retting high performance strain Dm111, the degumming rate and correlative parameter in fermentation broth was measured periodically in laboratory. The results indicated that quantity of viable organism and the degumming rate increased incessantly at prophase, then the trend became mild; the enzymatic activity of pectase and xylanase increased slowly at prophase, increased quickly at intermediate stage and degraded at anaphase. But the enzymatic activity of cellulase maintained stable and low in the whole retting course, pH change curve presented like ＂V＂ ; the curve change of reducing sugar shaped like a ＂M＂ in the whole retting peocess, that is, it had two peak values. The COD, proteins, residue in fermentation broth and retting time was positive correlation. The level of residue accounted for 27 % of hemp phloem.
出处
《纺织学报》
EI
CAS
CSCD
北大核心
2009年第3期48-52,共5页
Journal of Textile Research
基金
国家"十一五"支撑计划项目(2007BAD41B02
2006BAD06B03)
公益性行业科研专项项目(nyhyzx07-018)
国家948项目(2006G-18-2-2)
关键词
大麻
生物脱胶
脱胶菌
发酵液
酶活
hemp
microbial retting
retting strain
fermentation broth
enzymatic activity
