摘要
以广西桐棉和广西古蓬马尾松(Pinus massoniana Lamb.)种源叶片为材料,应用间接酶联免疫法(ELISA)测定内源激素脱落酸(ABA)、赤酶素(GA3)、吲哚乙酸(IAA)、玉米核苷(ZR)含量,分析不同种源苗期的抗寒性与内源激素含量的关系。结果表明,马尾松抗寒力的评价指标为内源GA3含量、ABA/GA3(k值)和ZR/ABA值,内源GA3含量越高,抗性越差;k值越大,抗寒力越强;ZR/ABA值越大,抗寒力越强。
The leaves of two improved provenances of Pinus massomana were taken as experimental materials of which the four endogenous hormones such as ABA, GA3, IAA, ZR were measured by Enyme-Linked Immunosorbent Assays （ELISA）. And the relationships between cold-resistance and endogenous hormones contents of seedlings from different provenances were analyzed combining with their growth traits. The research result indicates that the cold-resistance of Gupeng provenance is better than that of Tongmian provenance. Meanwhile, the cold-resistance indices of P. massoniana include the content of endogenous GA3, k, the ratio of ZR/ABA. And the higher the endogenous GA3 is, the worse the resistance is ; the cold-resistance increases with the ascending of k; furthermore, the increasing value of the rate of ZR/ABA can show the better cold-resistance.
出处
《广西科学》
CAS
2009年第1期87-91,共5页
Guangxi Sciences
基金
广西科学基金项目(桂科基0236036)
广西科技厅项目(桂科能0443001-9)资助
关键词
马尾松
种源
内源激素
抗寒性
Pinus massoniana, provenance, endogenous hormone, cold-resistance
