摘要
目的：对青老年人胃癌中ｐ１６ＩＮＫ４及ｒａｓｐ２１的表达进行研究，在分子生物学水平上探讨青老年人胃癌的发病学之异同。方法：应用过氧化酶标记的链霉卵白素（ＳＰ）染色法对６５例胃癌（其中青年组３５例，老年组３０例）进行免疫组化染色。由两位研究者对染色结果进行观察，采用已知的乳腺癌阳性切片作为对照。所有数据进行χ２检验。结果：在非选择人群，ｐ１６ＩＮＫ４和ｒａｓｐ２１在胃癌中的阳性表达率分别为３６．９％，７５．４％；ｐ１６ＩＮＫ４的阳性表达率随着胃癌分化程度的降低、浸润深度的加深而增高并与淋巴结转移有关；而ｒａｓｐ２１的阳性表达率随胃癌的分化程度降低而降低。ｐ１６ＩＮＫ４在青年人胃癌中的阳性率（６０％）显著高于老年人（１０％）（Ｐ＜０．０５），而ｒａｓｐ２１在前者的阳性率（６２．８％）则显著低于后者（９０％）（Ｐ＜０．０５）。结论：ｐ１６ＩＮＫ４表达的检测在判断胃癌的恶性程度及淋巴结转移趋势方面具有一定的参考价值。在判断胃癌的恶性程度方面，ｒａｓｐ２１具有一定的参考价值。青老年人胃癌的发病学不尽相同。
Purpose:To explore the pathogenesis differences between young and aged patients,examining the expressions of p16INK4 and ras p21 oncoproteins in gastric carcinomas.Method: Using streptavidin/peroxidase (SP)method,65 gastric carcinomas including 35 young and 30 aged patients were examined immunohistochemically.The stained sections were observed by two researchers using positively staining sections for p16 INK4 and ras p21 of breast carcinomas as contrasts.All data were tested by χ2 test.Results: In 65 gastric carcinoma cases,the positive rates for p16 INK4 and ras p21 were 36.9%,75.4% respectively. The positive rates for p16 INK4 increased as invasive depth increased and degree of differentiation of the gastric carcinoma decreased,and its positive rates in the cases with local lymph node mtastasis were dramatically higher than those without metastasis (P<0.05).The positive rates for ras p21 decreased as the degree of differentiation decreased. The positive expressions of p16 INK4 in young gastric carcinomas (60%) were higher than those in aged patients(10%)(P<0.05),but ras p21 positive rates (62.8%) in the young men were significantly lower than those in the old men (90%)(P<0.05).Conclusions:It will be useful to detect the expression of p16 INK4 in gastric carcinoma,on the part of assessing the malignant degree and lymph node metastasis of gastric carcinoma.Examining ras p21 overexpression in gastric carcinoma will be helful to evaluate its malignant degree.The pathogenesis about young and old gastric carcinomas were probably different.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1998年第1期1-3,共3页
Chinese Journal of Cancer
基金
河南省科委资助
