摘要
目的研究内蒙古地区蒙古族妇女维生素D受体基因（Vitamin Dreceptor，VDR）ApaⅠ多态性与骨密度（Bone mineral density，BMD）的关系。方法采用聚合酶链．限制性片段长度多态性（PCR—RFLP）分析技术检测内蒙古地区179例蒙古族妇女VDR基因ApaⅠ多态性，并用双能X线吸收仪检测上述人群无优势手臂骨密度。结果ApaⅠ多态性等位基因频率分布符合Hardy-Weinberg定律；基因型频率分布依次为aa：49．16％Aa：41．34％AA：9．50％。协方差分析显示，在所有受试者中VDRApaⅠ基因型与无优势手臂BMD无相关性。骨量减少组和骨量正常组间基因型和等位基因差异无显著性。结论内蒙古地区蒙古族妇女人群中，维生素D受体基因ApaⅠ多态性与骨质疏松症可能无相关性。
Objective To investigate the relationship between ApaⅠ Polymorphism of Vitamin D receptor（VDR） gene and bone mass in Mongol women of Inner Mongolia. Methods The VDR ApaⅠ genotype was determined by PCR-restriction fragment length polymorphism（RFLP） in 179 Mongol women, bone mineral density （BMD） at left forearm were measured using dual-energy X-ray absorptiometry（DEXA）. Results The allele frequencies of ApaⅠ polymorphism were in Hardy-Weinberg equilibrium. Frequencies distribution of VDR ApaⅠ genotype were aa： 49.16%, Aa： 41.34% and AA： 9.50%. Covariance analysis showed that no significant association between VDR ApaⅠ genotype and BMD at left forearm in all groups. There was no statistical significance between osteopenia group and normal bone mass group in gertotype and allele genes. Conclusion Among Mongol women in Inner Mongolia, there was no association between the ApaⅠ Polymorphism of VDR gene and osteoporosis.
出处
《中国骨质疏松杂志》
CAS
CSCD
2009年第3期189-192,共4页
Chinese Journal of Osteoporosis
关键词
维生素D受体
遗传多态性
骨密度
蒙古族妇女
Vitamin D receptor
Genetic polymorphism
Bone mineral density
Mongolian woman
