摘要
目的观察抑郁症模型大鼠学习记忆力及海马形态结构的变化。方法雌性SD大鼠20只,随机分为正常组和模型组,每组10只。正常组不给予任何处理,模型组给予孤养加21 d慢性不可预见性的温和应激(CUMS)。采用Open-Field、糖水消耗量及Morris水迷宫测试大鼠的行为学表现;光镜以及透射电镜观察大鼠海马组织结构。结果与正常组相比,模型组大鼠行为学测试显示其直立活动得分及水平活动得分均减少、糖水摄入百分比减少及学习记忆力下降;HE染色显示模型组大鼠海马锥体细胞层变薄,细胞间隙增大,排列紊乱,疏松,细胞结构不完整,甚至有大量细胞缺失;透射电镜下,模型组海马神经元较少,细胞核呈现皱缩改变,核膜凹凸不整模糊不清,并有局部断裂;胞质内线粒体肿胀变性,嵴消失。结论孤养加21 d CUMS可造成大鼠抑郁症模型;模型组大鼠学习记忆力下降可能与应激造成海马形态结构改变有关。
Objective To observe changes of learning and memory abilities and the ultrastructure of hippocampus in depression model rats. Methods Twenty female SD rats were randomly divided into two groups （10 in each） ： normal group and model group. The former was given no stress and the later was given isolation-housing in combination with 21 days of chronic unexpected mild stress（CUMS）. Open-Field test, sugar consumption and Morris water maze test were used to test rats＇ behaviors. Light Microscope and transmission electron microscope were used to observe the tissue structure of hippocampus. Results Comparison with rats in normal group, rats in model group showed changes in behavioral aspects： both crossing and rearing were lower than those in normal group in Open-Field test, the percentage of sugar consumption, as well as learning and memory abilities is too. HE staining showed that hippocampal pyramidal cell layer became thinner in rat of model group, the gap between cells increased, and the cells arrayed in disorder, loose, even a large number of cells were lost. Transmission electron microscope showed less hippocampal neurons in rats of model group, their nuclear collapsed, the whole nuclear membrane was fuzzy, and there were some local fractures in the nuclear membrane. Mitochondria within the cytoplasm were swelling and their ridges disappeared. Conclusion Depression model could be induced by isolation-housing in combination with 21 days of CUMS. The decline of learning and memory abilities in rats of model group may be caused by pathological changes in hippocampus.
出处
《江西医学院学报》
2009年第1期4-8,共5页
Acta Academiae Medicinae Jiangxi
基金
江西省自然科学基金(2007GQY0944)
关键词
慢性不可预见性的温和应激
海马
透射电镜
动物
实验
大鼠
chronic unexpected mild stress
hippocampus
transmission electron microscope
animals, laboratory
rats
