摘要
目的探讨不同浓度解脲脲原体血清型3（UU3）在小鼠生殖道中的致病性。方法将156只昆明小鼠按完全随机设计法分为A、B、C组（每组各48只）及对照组（12只）共4组。将3种不同浓度的UU3接种至各实验组小鼠阴道内，各组的浓度分别为：A组1×10^7copy／g，B组1×10^6copy／g，C组1×10^5copy／g；对照组仅接种UU液体培养基。于接种后第1、3、7、14、21、35天每次随机处死A、B、C组各8只及对照组2只小鼠，取宫颈管分泌物，荧光定量PCR（FQ—PCR）技术检测各组UU3的浓度及阳性率；取宫颈、子宫内膜及输卵管组织，光镜下观察其形态，并计算致病率。结果（1）A、B、C3组UU3总阳性率分别为63％（30／48）、50％（24／48）和17％（8／48），3组间比较，差异有统计学意义（P〈0．01）。接种后1、3、7、14、21、35d小鼠的UU3阳性率，A组分别为8／8、7／8、6／8、5／8、4／8和0，B组分别为7／8、5／8、5／8、4／8、3／8和0，C组分别为3／8、2／8、2／8、1／8、0和0；对照组各时间点均为0。接种后1d的UU3阳性率3组间比较，差异有统计学意义（P〈0．05）；其他各时间点3组间比较，差异均无统计学意义（P〉0．05）。（2）在UU3检测阳性者中，A、B、C组的UU3浓度在接种后1、3、7、14、21d分别为1．70×10^7、3．75×10^6和1．45×10^5copy／g，8．26×10^6、2．56×10^6和1．07×10^5copy／g，4．04×10^6、1．37×10^6和5．43×10^4copy／g，2．86×10^6、6．72×10^5和4．68×10^3copy／g，2．41×10^5、1．12×10^5和0copy／g。除接种后21d外，接种后各时间点A、B、C3组的UU3浓度比较，差异均有统计学意义（P〈0．05）；A、B组内接种不同时间点的UU3浓度比较，差异均有统计学意义（P〈0．05）；C组接种不同时间点的UU3浓度比较，差异无统计学意义（P〉0．05）。（3）接种后7～35d，A、B、C3组的总致病率分别为56％（18／32）、44％（14／32）和6％（2／32），3组间比较，差异有统计学意义（P〈0．01）；接种后7、14、21、35d小鼠的致病率，A组分别为5／8、5／8、4／8和4／8，B组分别为4／8、4／8、3／8和3／8，C组分别为1／8、0、I／8和0；对照组各时间点均为0。除接种后14d外，各时间点A、B、C3组间致病率比较，差异均无统计学意义（P〉0．05）。结论在小鼠生殖道局部接种不同浓度的UU3，其致病性不同。当UU3浓度≥1×10^6copy／g时，其致病性明显增强。
Objective To study the pathogenicity ot ureaplasma urealytlcum serotype 3 t u u3 ） yam different concentration in the genital tract of the mice. Methods A total of 156 Kunming mice were divided into 4 groups randomly, including group A, B, C （48 mice in every experimental group） and control group （ 12 mice in control group）. UU 3 at concentration of 1×10^7copy/g （group A）, 1×10^6copy/g （group B）, 1×10^5copy/g （group C） were inoculated into 48 mice in every experimental group intravaginally, in the mean time, culture medium of UU was given into 12 mice in control group. They were necropsied at 1, 3, 7, 14, 21, 35 days of postinoculation randomly, which included 8 mice of every experimental group and 2 mice of control group every time, and to detect UU3 expression from cervical secretions by FQ-PCR and observing the pathogenicity rate in tissues of cervix, endometrium, fallopian tube by light microscope and calculate the morbidity rate. Results （ 1 ） The total positive rates of UU3 were 63% （ 30/48 ） in group A, 50% （24/48） in group B, 17% （8/48） in group C, which showed a significant difference （ P 〈 0. 01 ）. And at 1,3,7,14,21,35 days of postinoeulation, the positive rates of group A were 8/8,7/8,6/8,5/8,4/8 and 0, group B were 7/8,5/8,5/8,4/8,3/8 and 0, group C were 3/8,2/8,2/8,1/8,0 and 0; all mice in control group were zero. At all time points, there were statistical difference in the positive rate among three experimental groups only at 1 day（P 〈0. 05 ）. （2） In the positive mice,their UU3 quantity concentration at 1,3,7,14,21 days were 1.70×10^7, 8. 26 ×10^6, 4.04 ×10^6, 2. 86 ×10^6, and 2.41×10^5 copy/g in group A; 3.75 ×10^6, 2. 56 ×10^6, 1.37 ×10^6, 6.72 ×10^5, and 1.12 ×10^5 copy/g in group B, and 1.45 ×10^5 , 1.07 ×10^5 , 5.43 ×10^4, 4. 68 ×10^3 , and 0 copy/g in group C. There were statistical difference among experimental groups at all time points except 21 days （ P 〈 0. 05 ）. Comparing the concentration among all time points of every group, both group A and B showed a significant difference（P 〈0. 05） ,group C didn＇t reach it（ P 〉 0. 05 ）. （ 3 ） The total pathogenicity rates of three groups were significant different at 7 - 35 days, which were 56% （18/32） in group A, 44% （14/32） in group B, 6%（2/32） in group C （P〈 0. 01 ）. And at 7,14,21,35 days of postinoculation, the pathogenicity rates in group A were 5/8,5/8,4/8 and 4/8 ,group B were 4/8,4/8,3/8 and 3/8, group C were 1/8,0,1/8 and 0; all mice in control group were zero, which demonstrated significant difference only at 14 days （ P 〈 0. 05 ） , no other statistical difference were observed （ P 〉 0. 05 ） . Condusions The pathogenicity of UU3 varies with different concentration in genital tract of mice. When UU3 concentration is more than 1 ×10^6 copy/g, the susceptibility to infection was intensified significantly.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2009年第3期204-208,共5页
Chinese Journal of Obstetrics and Gynecology
