摘要
从大庆某貉养殖场采集病貉的病理病料，处理后接种于犬肾细胞（MDCK），分离到8株病毒。对分离毒株进行了中和试验、血凝试验、理化性质的鉴定试验，应用PCR方法扩增分离毒株VP2部分基因并进行序列分析。结果显示，病料接种MDcK细胞72h后产生了明显的细胞病变（CPE）；分离毒株可以被犬细小病毒阳性血清中和，中和效价为1：10^6—1：10^7；分离毒株对氯仿、乙醚不敏感，耐酸耐热，可以凝集猪红细胞，其凝集作用可被犬细小病毒阳性血清抑制；用PCR检测病毒的细胞培养液，可扩增出长531bp的片段，该片段的核苷酸序列与吉林分离的犬细小病毒株（EU310373）的同源性为99.6％。表明，分离的这8株病毒均为貉源细小病毒。
Eight strains of virus were isolated from infected raccoon dogs from Daqing Prefecture in Heilongjiang Province by cultivation in Madin-Darby Canine Kidney（MDCK） cells. Then,the isolated viru ses were identified by the neutralization test, hemagglutination test, physico chemical property test. The results showed that the isolated viruses could cause typical cytopathogenic effect（CPE） in MDCK cells at hour 72 after infection. The isolated virus could be neutralized with the positive sera with canine parvovirus （CPV） at titers from 1：10^6 to 1：10^7 ,could be agglutinated by the porcine erythrocytes and be inhibited by the positive sera with CPV. The isolates were insensitive to chloroform,aether,acid and temperature. A DNA fragment of 531 bp was amplified by PCR from the MDCK cells infected with isolated virus. The homology of VP2 gene sequence between the isolated viruses and EU310373 from GenBank was 99.6%. It was concluded from the results that the 8 isolated viruses from raccoon dog represent parvovirus.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2009年第3期214-218,共5页
Chinese Veterinary Science
关键词
貉
细小病毒
分离
鉴定
raccoon dog（Nyctereutes procyonoides ）
parvovirus
isolation
identification
