摘要
目的：建立杏香兔耳风药材中绿原酸和3,5-O-二咖啡酰基奎宁酸的含量测定方法。方法：采用HPLC法,使用Dis-covery C18柱（250mm×4.6mm,5μm）,以乙腈-0.05%甲酸水溶液为流动相,流速1.0mL.min^-1,梯度洗脱,检测波长为328nm。结果：绿原酸进样量在0.60～3.00μg范围内呈线性关系（r=0.9996,n=5）,平均回收率为100.8%;3,5-O-二咖啡酰基奎宁酸进样量在1.30～6.52μg范围内呈线性关系（r=0.9997,n=5）,平均回收率为99.4%。结论：本方法操作简便、可靠,重复性好,专属性强,可作为杏香兔耳风药材质量控制方法。
Objective：To establish an HPLC method for determination chlorogenic acid and 3,5-O-dicaffeoylquinic acid in Ainsliaea fragrans Champ.Method：The chromatographic column was Discovery C18（250 mm×4.6 mm,5 μm）,UV detection wavelength was 328 nm,a mixture of 0.05% formic acid-water and acetonitrile was used as mobile phase with gradient elution and flow rate was 1.0 mL·min-1.Results：Chlorogenic acid at the quality of 0.60-3.00 μg showed good linearity,the average recovery（n = 5）was 100.8% ;3,5-O-dicaffeoylquinic acid at the quality of 1.30-6.52 μg showed good linearity,the average recovery（n=5）was 99.4%.Conclusion：This methods is fast,reliable and accurate,and may be as one of index used to judge the quality of Ainsliaea fragrans Champ.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第3期430-432,共3页
Chinese Journal of Pharmaceutical Analysis
