摘要
目的比较VITEK-32与VITEK2compact两种仪器对肠球菌鉴定的准确性。方法分别应用VITEK-32与VITEK2 compact对40株临床标本分离的肠球菌同时鉴定,用多重PCR技术对鉴定结果有差异的菌株进行种的检测并对产物测序。结果40株肠球菌中,32株用两种仪器鉴定结果完全相同,8株鉴定结果不一致。8株菌的PCR产物测序结果与GenBank中的屎肠球菌比较有97%～98%同源性,与VITEK2 compact鉴定结果完全相同。结论VITEK2 compact鉴定屎肠球菌的准确性优于VITEK-32。
Objective To compare the accuracy between VITEK-32 and VITEK-2 compact in identifying enterocei. Methods 40 strains of enteroeci isolated from various clinical specimens were indentified by VITEK-32 and VITEK-2 compact system respectively,the strains with different identification results were detected by multiple PCR,and the sequence of the products was mensurated. Results 32 isolates had consensus indentification,8 isolates had different identification.Nucleotide sequence analyzed for 8 isolates PCR products were 97-98% homologous compared with E.faecium of GenBank,which were agreed with identification using VITEK 2 compact system.. Conclusion The accuracy of identifying E.faecium in VITEK 2 compact system is better than that in VITEK 32 system..
出处
《中国医疗设备》
2009年第3期53-54,71,共3页
China Medical Devices
