摘要
目的针对目前体外培养成骨细胞方法的不足,探讨简便、经济、高效的原代成骨细胞培养方法.方法采用SD大鼠胎鼠颅盖骨培养原代成骨细胞,并收集1～3次连续植块进行培养,观察细胞形态、分裂增殖、碱性磷酸酶(ALP)活性及钙化的变化.结果连续植块培养收集的1～3次原代成骨细胞,在细胞贴壁、变形、铺展、汇合时间,细胞内碱性磷酸酶染色及矿化作用均无明显的区别.结论连续植块法用于培养SD大鼠成骨细包具有简便、经济高效的特点.
Objective To investigate a simple, economical and efficient method of primary osteoblast culture. Methods Primary osteoblasts of 1st-3th series explant culture from calvarial bones of foetusal Sprague-Dawley rats, were cultured in vitro. The identification was conducted by cell morphology, histochemieal staining of alkaline phosphatase （ALP） and calcified nodules staining. Results No significant difference was found in cell shape, spread, ALP activity and calcified nodules staining, which had been harvested from l st-3th series exptant culture of foetusal Sprague-Dawley rats. Conclusion This method of primary osteoblasts culture is practicable, efficient and effective.
出处
《昆明医学院学报》
2009年第2期32-35,共4页
Journal of Kunming Medical College
基金
云南省自然科学基金面上资助项目(2006C0001R)
