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bFGF真核表达载体的构建及其在MSCs中的稳定表达 被引量：1

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摘要 目的构建人碱性成纤维生长因子(bFGF)真核表达载体,观察其在人骨髓间充质干细胞(MSCs)内的表达。方法应用RT-PCR从人胎盘组织中扩增出bFGF,并将其构建于pcDNA3.1真核表达载体上。利用脂质体将基因转染MSCs内,利用G418进行基因的表达筛选,通过RT-PCR和Western blot进行基因表达的检测。结果扩增出人bFGF基因成功构建在bFGF-pcDNA3.1真核表达载体上。经G418筛选转染的MSCs存活十代以上。经RT-PCR及Western blot检测发现转染bFGF-pcDNA3.1真核表达载体的MSCs能够大量稳定表达bFGF。结论构建的bFGF-pcD-NA3.1真核表达载体可在MSCs内得到稳定的表达。
作者 杨春辉 杜珍武 卜丽莎 尹维田
机构地区 吉林大学第三临床医院手外科 吉林大学药学院基因工程教研室 吉林大学第三临床医院中心实验室
出处 《中国老年学杂志》 CAS CSCD 北大核心 2009年第6期697-699,共3页 Chinese Journal of Gerontology
关键词 碱性成纤维生长因子 骨髓间充质干细胞 基因稳定表达
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献12

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共引文献24

同被引文献30

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引证文献1

中国老年学杂志
2009年 第6期

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