摘要
目的研究不同浓度的山楂叶总黄酮对鹌鹑血脂和脂肪肝形成的防治作用。方法将60只雌、雄各半的鹌鹑分成6组:正常组(N)喂以普通饲料;高脂饮食模型组(M)喂以高胆固醇脂饲料,诱发形成脂肪肝模型;脂必妥药物对照组(ZBT)、山楂叶总黄酮低、中、高浓度实验组(HLF1、HLF2、HLF3)在喂高脂饲料的同时分别喂1g/kg体重ZBT、20mg/kg、40mg/kg、80mg/kg体重山楂叶总黄酮溶液各1mL。实验6个月后取动物血清、肝脏,检测动物血清、肝脏组织中总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷丙转氨酶(AST)、谷草转氨酶(ALT)的含量,制备鹌鹑肝脏组织石蜡切片和电镜切片观察其病理学变化。结果山楂叶总黄酮实验各组与模型组相比,其血清和肝脏匀浆液的TC、TG、LDL-C、MDA、AST、ALT等含量均显著下降(P<0.001),而HDL-C、GSH、SOD含量则明显上升(P<0.001),且山楂叶总黄酮的浓度呈明显的量效关系。石蜡切片和电镜切片也显示山楂叶总黄酮实验组动物肝脏中的脂质积累和脂肪肝病理学变化明显轻于模型组。结论山楂叶总黄酮具有明显降低血脂、减轻动物肝脏内各类脂质沉积的作用,还具有很好的抗氧化作用,能显著地保护肝脏组织的生理生化功能,对由高脂饮食引起的高血脂和脂肪肝具有明显的防治作用。
Objective To investigate the effect of hawthorn leaf flavonoids （HLFs） with the different concentrations in prevention of high blood lipids and fatty liver in quails. Methods Sixty quails were divided into six groups：normal （N） ,high lipids food model （M） ,positive control （ZBT） ,HLF1 ,HLF2 and HLF3 groups. The animals in the N group were fed with normal food,while the M group was feed with high lipids food to establish the animal model of fatty liver. Besides high-fat diet, HLFI, HLF2 and HLF3 groups were also fed with 1 mL HLFs in the doses of 20 mg/kg,40 mg/kg and 80 mg/kg respectively. The ZBT group was fed with l g/kg of ZhiBiTuo and used as the positive control. After the experiments of six months, the serum and liver of the quails were taken and used to evaluate the contents of total cholesterol （ TC）, triglyceride （ TG）, high density lipid cholesterol （HDL-C）, low density lipid cholesterol （ LDL-C）, superoxide dismutase （SOD）, malondialdehyde （ MDA）, glutathione （GSH） ,alanine amino transferase （ALT） and glutamie oxaloacetic transaminase （AST）. Meanwhile, the microscopic and electronic microscopic sections were used to observe the pathological changes of the livers of the quails. Results Compared with the M group, H LFs significantly lowered the content of TC,TG, LDL-C, MDA, AST and ALT in the serum and livers of the quails in other groups （P〈 0. 001）. However, the levels of HDL-C, SOD and GSH of the other groups were increased markedly （P〈0. 001 ）. Moreover, the ratios of increase/decrease in the three HLF groups were depended on the concentrations of HLFs. Also, better liver structure and less lipid accumulation were also observed in the HLF groups when compared to the M group. Conclusions These results showed HLFs can lower blood lipid and cholesterol accumulation, improve the antioxidant capacity and protect the physiological function of liver. So, HLFs were effective in preventing and treating high blood lipids and fatty liver induced by high-fat diet.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2009年第2期142-148,共7页
Fudan University Journal of Medical Sciences
关键词
山楂叶总黄酮
鹌鹑
血脂
脂肪肝
抗氧化作用
hawthorn leaf flavonoids
quail
blood lipid
fatty liver
antioxidant capacity
