摘要
利用"Lemont"和"Dular"水稻杂交后代单粒传衍生的123个F12家系所组成的重组自交系(Recombinant inbred lines,RILs)群体及其含97个SSR标记的连锁图谱,以耐性指数(T)和敏感性指数(S)为测定指标,应用WinQTLcart2.5定位软件,采用复合区间作图法对2个性状进行定位分析。结果表明,在RIL群体中,2个性状呈连续分布,受微效多基因控制,并且各性状均存在一定数量的超亲遗传类型。2个性状共检测到11个QTL,各QTL的LOD值为2.02~5.07,贡献率为6%~23%。其中在第1、2、3、6、8染色体上检测到控制耐性指标的7个QTLs,贡献率为6%~19%;在第1、3、5、8染色体上检测到控制敏感性指标的4个QTLs,贡献率分别为19%、23%、6%和7%;分别在第3、8染色体的相同区间内(RM85~RM468和RM408~RM250)检测到2个性状的QTLs,这很好地解释了2性状之间存在着极显著负相关性即存在一因多效现象(Pleiotrophic effect)。
A linkage map consisting of 97 SSR markers was constructed using 123 F12 lines (recombinant inbred lines, RILs) de- rived from crossing between "Lemont" and "Dular" rice cultivars. QTL analysis was conducted for flag leaf photooxidation tolerance, including tolerance index (T) and sensitivity index (S). Using WinQTLcart 2.5 software, a total of eleven QTLs are detected with percentage variance explanation (PVE) of 6%23%, and LOD of QTLs of 2.025.07. Of the QTLs, seven tolerance indices are de- tected on chromosomes 1, 2, 3, 6 and 8, with PVE of 6%19%; four controlling sensitivity indices are detected on chromosomes 1, 3, 5 and 8, respectively explaining 19%, 23%, 6% and 7% of total phenotypic variation. On the basis of both traits, two QTLs for toler- ance index and sensitivity index are co-localized around RM85RM468 and RM408RM250 respectively, which could explain negative significant correlation between tolerance index and sensitivity index, i.e . the pleiotrophic effect.
出处
《中国生态农业学报》
CAS
CSCD
北大核心
2009年第2期339-342,共4页
Chinese Journal of Eco-Agriculture
基金
国家自然科学基金项目(30571103
39970441)
教育部生态学博士点基金项目(20050389004)资助