摘要
目的观察维拉帕米对冻僵大鼠能量代谢和氧化代谢的影响。方法40只Wistar雄性大鼠随机分成5组。对照组在室温20℃下饲养。冻僵组为-30℃冷暴露80 min。干预1、2、3组分别依次预先30 min腹腔注射维拉帕米5、10、20mg/kg,然后于-30℃冷暴露80 min,室温复温4 h,麻醉后腹主动脉采血,测定血糖、乳酸(LD)、丙二醛(MDA)、谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)活力。摘取肝脏,测定肝脏中的MDA含量、GSH和SOD活力。结果冻僵组大鼠血糖低于对照组(P<0.05),冻僵组血清中LD、MDA、GSH和肝脏中LD,MDA、GSH含量及SOD活力均高于对照组(P<0.05)。3个干预组大鼠肛温温差、血糖、血清与肝脏中MDA含量和GSH活力均低于冻僵组(P<0.05)。干预2、3组血清中LD含量和肝脏中SOD活力均低于冻僵组(P<0.05)。结论维拉帕米不仅能够减轻能量代谢中无氧呼吸废物对机体的损伤,同时还能够降低冷暴露时对机体的氧化损伤。
[ Objective] To observe the effect of verapamil on the frozen stiff rats＇ energy and oxidation metabology. [ Methods ] 40 Wistar male rats were randomly divided into 5 groups. The control was raised in 20 ℃ room temperature. The frozen stiff group were dealt with - 30 ℃ temperature for 80 min. The intervening 1, 2 and 3 group were respectively pretreated intraperitoneaUy with 5mg/kg, 10mg/kg and 20mg/kg veripamil before the experiment 30 minutes earlier. Then the rats were treated with -30 ℃ temperature for 80min. After 4 hours recovered in the room temperature, blood was collected by puncture into abdominal aorta under anesthesia, then blood glucose, lactic acid （ LD）, malondialdehyde （ MDA ）, glutathione （ GSH }, superoxide dismutase （ SOD } were determined. Then the liver was plucked off, and MDA, GSH content and SOD vitality were determined. [ Results ] The blood glucose in the frozen stiff group is significantly lower than that in the control group （ P 〈 0.05 ）, and serum LD, MDA, GSH, and liver＇s LD, MDA, GSH level, SOD content in the frozen stiff group are all significantly lower than the control group （ P 〈 0. 05 ）. The anus temperature difference, blood glucose, serum, MDA content and GSH vitality in liver in all 3 intervening groups were significantly lower than that of the frozen stiff group. The LD content and the liver＇s SOD vitality in the 2, 3 intervening groups are significantly lower than the frozen stiff group. [ Conclusion] The verapamil can not only abate the waste＇s injury to the rats in the anaerobic respiration metabology, but also reduce the oxidation injury due to cold exposure.
出处
《职业与健康》
CAS
2009年第7期676-678,共3页
Occupation and Health
关键词
维拉帕米
冻僵
代谢
Verapamil
Frozen stiff
Metabology
