摘要
目的探讨空间结构对真皮组织成纤维细胞的形态及生物学行为的影响。方法采用微纳制造技术设计并制作细胞调控点,微接触印刷术制备基膜,细胞接种后分别行细胞形态学观察;免疫组化检测细胞α-平滑肌肌动蛋白(α-SMA)表达;MTT试验分析细胞活力;ELISA法测定细胞培养上清中羟脯氨酸含量。以单纯细胞培养皿培养的细胞作为对照组。结果与对照组比较,经细胞调控点调控的细胞形态多样,呈现类圆形,伪足更长;α-SMA表达上调;细胞活力降低;细胞培养上清中羟脯氨酸含量显著增加;统计学分析表明,组间比较差异均有统计学意义(P<0.05)。结论细胞生物学行为呈现结构依赖性,空间结构可调控细胞生物学行为。
Objective To explore the effects of three-dimensional structure on the morphology and biological behavior of fibroblasts of dermal tissues. Methods Substrates were prepared by microcontact printing with special array of microdots designed and made by micro/nano processing technique. After dermal fibroblasts were cultured on the substrates,cell morphology was observed,expression of α-smooth muscle actin (α-SMA) was detected by immunohistochemistry,cell viability was examined by MTT,and contents of hydroxyproline in the supernatant were determined by ELISA. Cells cultured on pure cell culture plates were served as controls. Results Compared with controls,the regulated cells exhibited diverse morphology,with spherical-like shape and longer pseudopodia. The expression of α-SMA was up-regulated,cell viobility was down-regulated,and contents of hydroxyproline increased significantly (P〈0.05). Conclusion The cell biological behavior is structure-dependent,which can be regulated by three-dimensional structure.
出处
《上海交通大学学报(医学版)》
CAS
CSCD
北大核心
2009年第3期312-315,共4页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家重点基础研究发展计划(“973”计划) (2005CB522603-3)
国家自然科学基金(30872685)~~
关键词
空间结构
细胞调控
微接触印刷术
细胞生物学行为
three-dimensional structure
cell regulation
microcontact printing
cell biological behavior