摘要
将300条斑马鱼随机等分为10组和空白对照组。水浴染毒5 d后,采用分光光度法测定其肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽-S-转移酶(GST)的活力,研究TCDD和PCB77对斑马鱼肝脏MDA、SOD、GST的影响。结果发现,TCDD染毒5 d后,各剂量染毒组MDA含量均有所增加,其中0.2μg/L组差异显著(P<0.05),0.4μg/L和0.8μg/L剂量组差异极显著(P<0.01);各染毒组SOD活性均有所下降,其中0.2、0.4和0.8μg/L剂量组差异极显著(P<0.01);各染毒组GST活力均降低且差异极显著(P<0.01)。PCB77染毒5 d后,各剂量染毒组MDA含量均有所增加,其中0.2 mg/L组差异显著(P<0.05)、0.4 mg/L和0.8 mg/L组差异极显著(P<0.01);各剂量染毒组SOD活力均有所下降,其中0.2 mg/L组差异显著(P<0.05),0.4 mg/L和0.8 mg/L组差异极显著(P<0.01);各剂量染毒组GST活力也均有所下降,其中0.2、0.4和0.8 mg/L组差异极显著(P<0.01)。可见,一定剂量的TCDD和PCB77能分别引起MDA含量增加,使SOD和GST活力降低,对斑马鱼具有脂质过氧化作用。
In this manuscript, in order to investigate the effects of 2,3,7,8-tetrachlorodibenzo-pdioxin （TCDD） and 3,3＇, 4,4＇-tetrachlorobiphenyl （PCB77） on hepatic malondialdehyde （MDA）, superoxide dismutase （SOD） and glutathionetransferase （GST） in zebrafish, 300 zebrafish were randomly divided into 10 groups： Four test groups exposed to TCDD at 0. 1, 0.2, 0. 34, 0.8 μg/L and control group. Four test groups exposed to PCB77 at 0.1, 0.2, 0.34, 0.8 μg/L and control group. Five days after the animals were exposed to the polluted water with TCDD or PCB77, the MDA level and the SOD/GST activities in zebrafish liver were measured by spectrophotometrically. It was found that the MDA level and SOD activity increased in which TCDD was more than 0.2 12g/L （P〈0. 05）. The GST activity increased in all the groups. Furthermore, the MDA level increased in which PCB77 was more than 0.2 μg/L （P〈0.05） while the SOD activity decreased in the conditions （P〈0.01）. It was concluded that TCDD and PCB77 at certain doses could cause hepatic lipid peroxidation in zebrafish by induction of MDA increase and SOD/GST inactivation.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2009年第2期210-213,共4页
Journal of Food Science and Biotechnology
基金
广东省自然科学基金项目(05011789)
广东省国际合作项目(2007B050200023)
关键词
2
3
7
8-四氯代二苯并二噁英
3
3’
4
4’-四氯联苯
丙二醛
超氧化物歧化酶
谷胱甘肽-
S-转移酶
斑马鱼
2,3,7,8-Tetrachlorodibenzo-p-dioxin, 3,3＇, 4,4＇-tetrachlorobiphenyl, malondialdehyde, superoxide dismutase, glutathionetransferase, zebrafish
