摘要
目的探讨釉基质蛋白对牙髓细胞增殖的作用及其机理。方法分离培养人的牙髓细胞,加入不同浓度的Emdogain,采用化学发光免疫测定方法比较牙髓细胞的生长情况,采用Superarray方法研究加入Emdogain对细胞周期相关因子的影响。结果牙髓细胞在不同浓度的Emdogain作用下呈现不同的生长速率。Emdogain促进牙髓细胞生长的最佳浓度为300μg/ml。细胞周期相关因子的Superarray分析显示,Emdogain通过上调cyclin D1,p21,E6-AP,SUMO-1基因达到对细胞生长的促进作用。结论适宜浓度的Emdogain(45～600μg/ml)能够促进牙髓细胞的增殖。
Objective To investigate the effect and mechanism of enamel matrix proteins on the proliferation of dental pulp ceils. Methods Dental pulp cells were isolated and cultured. Emdogain at various concentrations was added to the media, and the rate of cell proliferation was compared with the control cells by chemiluminescence immunoassay. The effect of Emdogain on cell proliferation was further explored by cell cycle pathway - specific superarray analysis. Results Dental pulp cells showed different proliferation rates at varying concentrations of Emdogain. The highest cell proliferation rate was found at 300μg/ml of Emdogain. Cell cycle pathway - specific superarray analysis showed that Emdogain enhanced pulp cell proliferation by upregulation of cyclin D1, p21, E6 - AP, and SUMO - 1. Conclusion Emdogain at optimal concentrations （45 - 600 μg/ml ） increased DPSCs proliferation.
出处
《现代口腔医学杂志》
CAS
CSCD
2009年第2期162-164,共3页
Journal of Modern Stomatology
基金
国家自然科学基金面上项目(30772419)
