摘要
目的:初步探讨B淋巴细胞激活因子受体(Bcells-activating factor receptor,BAFFR)在胚胎植入调控中的作用。方法:选取正常早孕妇女人工流产蜕膜组织,分离、提纯早孕蜕膜基质细胞(decidual stromal cells,DSC),培养于含10%去激素胎牛血清培养基内,分为雌二醇(E2)组、孕酮(P4)组、E2+P4组和空白对照(C)组,培养96h。MTT检测细胞的增殖能力;Real-time PCR检测BAFFR mRNA;流式细胞仪检测BAFFR蛋白表达。结果:与C组比较,各实验组DSC增殖能力及BAFFR mRNA水平均无明显变化(P>0.05);BAFFR蛋白定位于DSC胞内,且E2+P4可显著上调其表达(P<0.05)。结论:正常人早孕DSC表达高水平的BAFFR,雌、孕激素联合对其有显著的上调作用,提示BAFFR可能参与胚胎植入的调控及早孕期母-胎界面的免疫调节。
Objective： To explore the role of B cells-activating factor receptor （BAFFR） in the embryo implantation. Methods： Human decidual stromal cells （DSC） isolated from the human deciduas of early pregnancy were purified and cultured in medium supplemented with 10% dextran-coated charcoal-treated fetal bovine serum. Then the cells were cultured in the same medium in absence or presence of E2, P4, and E2＋P4 for 96 h. The cell proliferation of DSC was verified by MTT. The transcription and translation levels of BAFFR gene were analysed by Real-time PCR and flow cytometry, respectively. Results： There were no significant differences in cell proliferation among the 4 groups, and the expression levels of BAFFR mRNA exhibited the same result as cell growth. However, the intracellular BAFFR protein levels were up-regulated significantly by P4＋E2（P〈0.05）. Conclusion： These results suggest that up-regulation of intracellular BAFFR protein levels in human DSC by E2＋P4 may participate in the process of embryo implantation and immuned regulation at the maternal-fetal interface.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2009年第3期157-161,共5页
Reproduction and Contraception
基金
国家自然科学基金资助项目(30872321
30671902)
