摘要
目的：利用在碱性溶液中硫胺素（VB1）与过氧化苯甲酰（BPO）的荧光反应,建立一种荧光光度法测定药品中硫胺素的新方法。方法：在碱性条件下,BPO将硫胺素氧化成具有较强荧光的硫胺荧,用荧光分光光度计（λex=371 nm,λem=440 nm）测定溶液荧光强度F值,与试剂空白溶液F0比较,以ΔF对硫胺素浓度进行线性回归。同时对体系的反应条件进行了一系列的研究。结果：硫胺素浓度在0.80×10^-5～0.64×10^-4mol/L范围内,体系荧光强度变化（ΔF）与其呈现良好线性关系,线性回归方程为：ΔF=39.768C（×10^-4mol/L）＋0.4117,检出限为0.24×10^-5mol/L。对11个浓度为0.4000×10^-4mol/L的硫胺素溶液进行测定,相对标准偏差为1.43%。应用于药物中硫胺素的测定,回收率为94.2%～103.8%。结论：此方法简便、快速,可用于药品中硫胺素的测定。
Objective：To establish a new method for determining thiamine based on the fluorimetric reaction of thiamine with benzoic peroxide in alkaline solutions.Methods：The method was based on the fluorimetric reaction of thiamine with benzoic peroxide in alkaline solutions.And under optimal conditions,the enhanced fluorescence intensity（ΔF=F-F0） had good linear correlation with the concentration of thiamine at 371 nm（λex） and 440 nm（λem）.Results：The results showed that the determination range was between 0.80×10^-5 and 0.64×10^-4 mol/L,the linear regression equation ΔF=39.768C（×10^-4mol/L）＋0.4117 and the detection limit 0.24×10^-5 mol/L.And the relative standard for 0.4000×10^-4 mol/L thiamine was 1.43%（n=11）.The method can be applied to the determination of thiamine in commercial pharmaceutical preparations and the recoveries were between 94.2% and 103.8%.Conclusion：The method of determining thiamine is simple,rapid and accurate.
出处
《中国卫生检验杂志》
CAS
2009年第3期515-516,共2页
Chinese Journal of Health Laboratory Technology
