摘要
人工核酸酶在基因药物设计和分子生物学研究领域具有重要的科学意义,因此人工核酸酶的设计和酶模拟方面的研究引起了广泛关注并取得了重要进展.本文介绍近年来国内外学者和作者研究组在"双功能协同催化"、"双核协同催化"、"无金属催化"的人工核酸酶方面的研究进展,尤其是氮杂冠醚衍生物作为人工核酸酶与DNA相互作用的研究进展.
The design of artificial nucleases and nuclease mimics has attracted extensive attention and made great progress due to their significant scientific meanings and potential application in the field of gene medicine and molecular biology. This paper reviews recent progress in the investigation of artificial nuclease, including ＂bifunctional cooperative catalysis＂, ＂dinuclear synergistic catalysis＂, ＂metal-free catalysis＂, and especially, the studies of aza-crown ethers as artificial nucleases and their interaction with DNA.
出处
《中国科学（B辑）》
CSCD
北大核心
2009年第3期194-207,共14页
Science in China(Series B)
基金
国家自然科学基金(批准号:20872061
20372032)
国家重点基础研究发展计划(编号:2007CB925103)项目资助
关键词
人工核酸酶
DNA切割
无金属切割DNA
胍基
氮杂冠醚
artificial nuclease, DNA cleavage, metal-free cleavage DNA, guanidinium group, aza-crown ether
