摘要
为了研究不同培养时间对家猫卵母细胞核成熟的影响,以M199为基础培养液,并添加FSH、LH,分别培养24、28、32、36和43h后,观察第一极体排出,然后用地衣红染色,将排出第一极体和染色体处于MⅡ期作为卵母细胞成熟的参考判断标志。结果表明,家猫卵母细胞随着培养时间的延长其成熟率也随之增加。成熟培养43h,极体排出率极显著高于其他各组(P<0.01),达到66.89%;而24h组极体排出率为31.51%,极显著低于其他各组(P<0.01);28、32、36h各组间差异不显著(P>0.05)。经地衣红染色后,到达MⅡ期的卵母细胞比例与极体排出率趋势相同。43h组退化率极显著高于其他各组(P<0.01),达到22.30%。因此,综合考虑成熟率和退化率两项指标,28～36h为家猫卵母细胞体外核成熟的最佳培养时间。
The aim of this study is to investigate the effect of different durations on nuclear maturation in vitro of feline oocytes. Following the in vitro maturation （ⅣM） in Medium 199 added with FSH and LH, feline oocytes were treated for 24 h, 28 h, 32 h, 36 h and 43 h respectively. Then, the polar body 1（PB1） was observed and oocytes were stained by aceto-orcein solution, and those with PB1 and stayed at M Ⅱ phase, were considered as nuclear maturation. The rate of nuclear maturation in the 43 h group was significantly higher than the others （P〈0. 01）. The rate of nuclear maturation in the 24 h group was significantly lower than the others （P〈0.01）, but there was no significant differences of the rate of nuclear maturation among other three groups （P〉0.05）. In addition, the rate of degeneration in the 43 h group was also significantly higher than the others （P〈0.01）. Therefore, the optimal nuclear maturation of feline oocytes appeared following the duration from 28 to 36 h by ⅣM.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第3期102-106,共5页
China Animal Husbandry & Veterinary Medicine
基金
北京市教育委员会科技发展计划重点项目(KZ200510020013)
北京市教育委员会科技发展计划面上项目(KM200710020006)
2007年度教育部留学人员科技活动择优资助项目(启动类)
关键词
家猫
卵母细胞
体外核成熟
培养时间
domestic cat
oocyte
in vitro nuclear maturation
duration
