摘要
对蛋白酶A的荧光检测方法进行了研究。分析了标准蛋白酶A稀释液对标准曲线建立的影响、检测方法的稳定性及检测时间对样品结果的影响。并对蛋白酶A酶活力与荧光值的相关性进行了分析,证明了在同一批样品检测中蛋白酶A酶活与对应的荧光值具有很好的相关性,可以根据荧光值的大小直接判断和比较蛋白酶A活力的高低。
In this paper, the fluorescent detection method of protease A was studied. The effects of standard protease A dilution on the establishment of standard curve were analyzed, and the effects of the detection method stability and detection time on sample testing results were investi- gated. Besides, the relevance ofprotease A activity and fluorescence values was analyzed. The results showed that protease A activity in the same batch of samples had good correlations with the corresponding values of the fluorescence. Accordingly, protease A activity could be judged and compared based on the values of fluorescence.
出处
《酿酒科技》
2009年第4期108-110,113,共4页
Liquor-Making Science & Technology
基金
"十一五"国家科技支撑计划项目(2007BAK36B00)"我国优势传统食品啤酒制造业关键技术研究与应用"子课题--纯生啤酒泡沫稳定性研究与应用
关键词
纯生啤酒
蛋白酶A
荧光底物
draft beer
proteinase A
fluorescent substrate
