摘要
从土样中分离筛选链霉菌4301菌株的代谢产物,固体培养4301菌株.结果表明,4301菌株对荔枝霜疫霉菌Peronophythora litchii和荔枝炭疽病菌Glomerella cingulata的抑菌圈透明,对荔枝霜疫霉菌的抑菌圈直径达到25.5mm.以荔枝霜疫霉菌为指示菌,对菌株4301发酵培养基和发酵条件进行优化研究,得到的最佳培养基组成(w)为:葡萄糖2%、可溶性淀粉2%、黄豆饼粉浸液3%、蛋白胨0.3%、FeSO40.001%、NaCl 0.2%、CaCO30.25%、K2HPO40.02%;优化后的培养条件:发酵时间为96 h、发酵温度为28℃、接种量(w)为8%、装液量为75 mL,优化后4301菌株对荔枝霜疫霉菌的抑菌圈直径达到28.8 mm.
Streptomyces strain 4301 was isolated from the soil. Metabolites produced by this strain showed fungicidal activity. The diameter of inhibitory cycles of Peronophythora litchii affected by Strcptomyces strain 4301 was 25.5 mm in solid culture. Optimal fermentation conditions and medium of liquid culture for Streptomyces strain 4301 were investigated. The results indicated that the optimal fermentation medium and conditions were as follows： glucose 2% , soluble starch 2% , beancake powder extract 3% , protose peptone 0.3% , FeSO4 0. 001%, NaC1 0.2%, CaCO3 0.25% , K2HPO4 0.02% ; inoculation volume 8% for 75 mL fermentation medium in 500 mL flask, culture temperature 28℃ and period 96 h. The diameter of inhibitory cycles of P. litchii affected by Streptomyces strain 4301 was 28.8 mm in the optimum medium and optimum fermentation conditions.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2009年第2期15-18,共4页
Journal of South China Agricultural University
基金
国家自然科学基金(30670331)
广东省自然科学基金重点项目(04105977
8451064201001012)
广东省科技计划项目(2008B021500001)
关键词
链霉菌
抑菌活性
发酵条件
荔枝霜疫霉菌
Streptomyces
fungicidal activity
fermentation conditions
Peronophythora litchii
