摘要
研究了斜卧青霉原生质体制备和再生的条件,确定了培养方式、菌龄、渗透压稳定剂、酶的配比、酶解时间等因素对原生质体制备和再生的影响。最佳条件:菌丝体培养12h,用1%溶壁酶+1%纤维素酶+1%蜗牛酶的混合酶液酶解,将NaCl作为渗透压稳定剂,酶解时间为1.5h。在此条件下原生质体的形成量达到5.3×105个/mL,再生率为19.4%。
Conditions of preparation and regeneration of PeniciUium decumbens protoplast, such as culturing conditions, incubation time, osmotic stabilizer and enzyme system were studied. The optimal conditions are shown as follows： the myeelia were cultured for 12 hours and suspended in 0.7 mol/L NaCl, and then wall-solving enzyme （ 1% ）, cellulase （ 1% ） , and snail enzyme （ 1% ） were added to it, NaC1 was used as osmotic stabilizer, the time of enzymolysis was 1.5 hours. Under these conditions the formation of protoplast reached up to 5.3 × 10^5/mL, and the regeneration rate was at 19.4%.
出处
《微生物学杂志》
CAS
CSCD
2009年第1期18-21,共4页
Journal of Microbiology
基金
国家自然科学基金资助项目(10502004)
关键词
斜卧青霉
原生质体制备
原生质体再生
Penicillium decumbens
protoplast preparation
protoplast regeneration
