摘要
以ＰＥＧ／硫酸铵双水相体系，经一次萃取从α－淀粉酶发酵液中分离提取α－淀粉酶和蛋白酶．萃取最适宜的条件为１５％ＰＥＧ１０００、２０％（ＮＨ４）２ＳＯ４，ｐＨ＝８，α－淀粉酶收率９０％，分配系数为１９．６；与蛋白酶的分离系数高达１５．１，比活率为原发酵液的１．５倍，蛋白酶在水相中的收率高于６０％．本文对反萃取条件进行了初步研究．与传统的提取法相比，本法可直接处理发酵粗滤液，提高工效，降低能耗和酶活损失．
Aqueous two phase system composed of PEG/ (NH 4) 2SO 4 was once to isolate α amylase and proteinase from fermented liquor of α amylase in this paper. The optimum extraction conditions were: 15% PEG1000, 20% (NH 4) 2SO 4 , pH=8, the partition coefficient of α amylase was as high as 19.6 with a yield of 90%. The separation coefficients between α amylase and proteinase were 15.1 and 1.5 folds increase of specific activity compared with those the original fermented liquor. The yield of proteinase in water phase was more than 60%. The preliminary study on back extraction condition was done in this paper.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1998年第3期391-394,共4页
Chemical Journal of Chinese Universities
基金
江苏省青年科技基金
关键词
蛋白酶
双水相
淀粉酶
萃取
反萃取
Α-淀粉酶
α Amylase, Proteinase, Aqueous two phase system, Polyethylene glycol, Extraction, Back extraction
