摘要
目的:建立藤黄健骨胶囊中淫羊藿苷的含量测定方法。方法:采用高效液相色谱法测定,用C18柱(150mm×4.6mm,5μm),以乙腈∶水(27∶73)为流动相,检测波长270nm。结果:淫羊藿苷的线性范围为0.1500～1.000μg;相关系数r=0.9999,平均回收率为99.33%,RSD=0.63%(n=5)。结论:所用方法测定样品灵敏度高,重复性好,能有效地控制藤黄健骨胶囊的质量。
Objective：To establish method for the determination of Icraiin in Tenghuang Jiangu Jiaonang; Methods：The separation was carried out on C18（ 150× 4.6mm, 5μm） column ; The mobile phase was composed of acetonitrile-H2O（ 27 ： 73 ）, Detection wavelength was 270nm. Results： The linear range of Icraiin was 0.1500 - 1.000μg （ r = 0.9999） ;Average recovery was 99.33% （RSD = 0.63% ; n = 10）. Conclusion：This method was accurate,rapid,reproducible and suitable for the quality control of this preparation.
出处
《光明中医》
2009年第4期646-647,共2页
GUANGMING JOURNAL OF CHINESE MEDICINE
基金
湖南省教育厅资助科研项目(No:08C663)
怀化学院资助科研项目(No:2007361)
