摘要
[目的]为检测动物性食品中的三聚氰胺提供参考方法。[方法]以鸡肉匀浆为材料,用高效液相色谱法和气相质谱法检测其中的三聚氰胺含量,并对检测方法的回收率和精密度进行测定。[结果]高效液相色谱法确定的鸡肉中三聚氰胺的最低检测限为1μg/g,最低定量限为2μg/g,在0.05-50μg范围内线性关系良好;方法的回收率大于80%,测定结果的日内变异系数小于7%,日间变异系数小于10%。气相质谱法确定的三聚氰胺的最低检测限为30ng/g,最低定量限为5030ng/g,在5—500ng/g范围内线性关系良好;方法的回收率在80%以上,测定结果的日内变异系数小于8%,日间变异系数小于10%。[结论]该研究为鸡肉中三聚氰胺的检测提供了新方法。
[ Objective ] The study was to provide a reference method for detecting melamine in animal food. [ Method ] With the homogenate of chicken meat as the material, the melamine amount in it was detected with high performance liquid chromatography ( HPLC ) and gas phase mass spectrometry method, and then the recovery rate and the precision of the 2 detection methods were determined. [ Result] The minimum detection limit on melamine in chicken meat by high performance liquid chromatography (HPLC) was 1 μg/g, and the limit quantification was 2 μg/g, and in 0. 05 -50 μg/g, the linear relationship was good. The recovery rate of this method was over 80%, the within-day variation coefficient was less than 7%, and the inter-clay variation coefficient was less than 10%. The minimum detection limit on melamine in chicken meat by gas phase mass spectrometry method was 30 ng/g, and the limit quantification was 5 030 ng/g, and in 5 -500 ng/g, the linear relationship was good. The recovery rate of this method was over 80%, the within-day variation coefficient was less than 8%, and the inter-day variation coefficient was less than 10%. [ Conclusion]The study provided a new method to detect melamine in chicken meat.
出处
《安徽农业科学》
CAS
北大核心
2009年第8期3361-3363,共3页
Journal of Anhui Agricultural Sciences
关键词
三聚氰胺
高效液相色谱
气相质谱
Melamine
High performance liquid chromatography(HPLC)
Gas phase mass spectrometry