摘要
对同一地区两例输血后丙型肝炎进行ＰＣＲ分型，结果为Ⅱ型。分别将其ＮＳ５区基因部分片段克隆到ｐＵＣ１８和Ｍ１３ｍｐ１８中，序列分析结果表明在ＮＳ５区基因相同区段，二者的核苷酸同源性为８９．０４％，氨基酸同源性为９０．７５％。而且在分离株Ｖ中第７０～７２位发现阅读框内终止密码子ＴＧＡ。与Ⅱ型代表株ＨＣＶＢＫ序列相比，相同区段的核苷酸和氨基酸同源性分别为：９１．５％，９１．９２％和９１．９１％，９１．９１％。对比分析表明，在同一地区基因型相同的不同分离株间ＮＳ５区基因仍存在较大变异。
Isolates from two patients of hepatitis C in the same region were classified as genotype Ⅱ by PCR method. NS5 gene fragments of HCV were cloned, and inserted into pUC19 and M13mp18, respectively.Sequence analysis showed that the homologies of the two isolates (NS5X and NS5V) in nucleotide and amino acid are 89.4% and 90.75%,respectively. Stop codon TGA was found in NS5V at 70～72 nt. The homologies of nucleotide and amino acid between the two isolates and typical genotype Ⅱ(HCV BK)are 91.5%and 91.92%,91.91% and 91.91%. The results indicated that there is relatively high variation in HCV NS5 region between isolates of same genotype from same area, and suggested that the isolate of NS5V may be a defective virus.
出处
《中国病毒学》
CSCD
1998年第1期50-56,共7页
Virologica Sinica
基金
国家863资助项目
