摘要
目的 :探讨健脾益气方 (JPYQR)抑制胃粘膜损伤的作用机理。方法 :采用束缚水浸法造成大鼠急性胃粘膜损伤 ,测定溃疡指数 ,检测血浆及胃组织中热休克蛋白 (heatshockprotein ,HSP70 )的变化 ,并对胃组织细胞HSPs进行免疫组织化学研究。结果 :用中药JPYQR和西药丽珠得乐预处理的大鼠 ,溃疡指数小于模型组 (P <0 0 0 1 ) ,而JPYQR组又小于丽珠得乐组 (P <0 0 1 ) ;预处理两组血浆及胃组织中HSP70 水平相反明显高于模型组 (P <0 0 5 ) ,而JPYQR组血浆中HSP70 水平又显著高于丽珠得乐组 (P <0 0 5 ) ,二者胃组织中的HSP70 水平虽有差异 ,但无统计学意义 (P >0 0 5 ) ;免疫组织化学研究提示腺体表达大量HSP70 和HSP60 ,按模型组、丽珠得乐组、JPYQR组依次增强。结论 :中药健脾益气方能诱导大鼠提高HSPs的表达 ,防止胃粘膜损伤 ,HSPs可能介导胃粘膜防御。
Objective: To investigate the mechanism of Jianpi Yiqi Recipe (JPYQR) in inhibition of gastric mucosa injury. Methods: A rat model of acute injury of gastric mucosa was established by binding and immersing in water. Ulcer index (UI) was measured, change of heat shock protein (HSP 70 ) in plasma and gastric tissue was examined, and the gastric HSPs was studied immunohistochemically. Results: The UI in rats pretreated with JPYQR or Lizhudele was lower than that of model group (P<0 001), and UI of JPYQR group was lower than that of Lizhudele group (P<0 01). On the other hand, the HSP 70 level in the two pretreated groups were significantly higher than that of the model group (P<0 05), and the plasma HSP 70 level of JPYQR group was higher than that of Lizhudele group (P<0 05). The difference of gastric HSP 70 level between the two pretreated groups was insignificant (P>0 05). Immunohistochemical study revealed a high level expression of HSP 70 and HSP 60 by glands and the level of the expression in model group was the highest, followed by those in Lizhudele group and JPYQR group. Conclusion: JPYQR could enhance the expression of HSPs in rats, and so as to prevent the gastric mucosa from injury. HSPs might involve in the defending mechanism of gastric mucosa.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1998年第3期162-164,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家中医药管理局资助课题! (No .95B15 1)
关键词
健脾益气方
热休克蛋白
胃粘膜损伤
Jianpi Yiqi Recipe, heat shock protein, acute injury of gastric mucosa
