摘要
利用5.8S核糖体DNA全长间隔序列(ITS/5.8S)和叶绿体基因matK对中国原产诸葛菜复合群的系统发育关系进行了分析研究。ITS序列结果支持中国诸葛菜复合群分为两支:一支由湖北诸葛菜和太白诸葛菜组成;另一支由诸葛菜和铺散诸葛菜组成。matK的序列分析结果表明,在中国诸葛菜复合群的系统发育树中,铺散诸葛菜和诸葛菜处于基部位置。结合以前的核型分析和本文的研究结果,我们不支持将湖北诸葛菜和太白诸葛菜归隶于诸葛菜,而支持我们之前提出的两种可能的原始细胞型的进化假说。另外,根据生物地理学分析,推测从长江中下游到秦岭地区是中国原产诸葛菜的近代分化中心。
A first insight into the phylogenetic analysis of the Orychophragmus violaceus complex was constructed from complete sequences of nuclear ribosomal DNA internal transcribed spacer regions （ITS/5.8S） and chloroplast gene matK. The ITS results strongly supported the division of the O. violaceus complex into two major groups： one is composed of O. hupehensis, O. taibaiensis, the other consists of O. violaceus and O. diffusus. The mark phylogenetic trees showed O. diffusus and O. violaceus were the basal members of the complex. From previous karyotype study and the present results, we considerated that the groups of O. hupehensis and O. taibaiensis should not be allocated in O. violaceus. And two possible ancestral cytotypes were also confirmed by our phylogenefic analysis. Moreover, the biogeographic analysis implied a prestuned center of recent differentiation in the region from upper to mid Changkiang River to Qin Mountain.
出处
《云南植物研究》
CSCD
北大核心
2009年第2期127-137,共11页
Acta Botanica Yunnanica
基金
National Natural Science Foundation of China (30570968
30671165)
Key Project of Ministry of Education (105140)
