摘要
根据抑制缩减杂交文库获得的香蕉谷氨酸脱羧酶基因片段,利用RACE技术,首次从香蕉果实中克隆了谷氨酸脱羧酶基因的cDNA全长.结果表明,该cDNA的ORF全长1 500 bp,编码499个氨基酸.Blast分析表明,该基因所推导的氨基酸序列与水稻、柑橘、白杨、番茄等具有较高的一致性,分别为82%、81%、79%、78%,推测其编码的蛋白质分子量为56.25 kD,等电点5.21,具有与钙调蛋白结合的C端延伸区域和磷酸吡哆醛结合位点.组织特异性和果实采后正常成熟不同阶段表达结果显示,该基因在香蕉根、茎、叶、花、果实中均有表达,果实中的表达量较高,正常成熟表达量的增加可能受内源乙烯诱导并促进乙烯生物合成,推测其可能在不同的生理过程中起作用,并且与果实成熟及乙烯生物合成密切相关.
The GAD gene was isolated from banana fruit （Musa acuminata L. AAA group cv. Brazilian） and designated as MaGAD1. The full length of MaGAD1 cDNA is 1 500 bp with an open reading frame encoding 499 amino acids that shared high identities with the GAD from Oryza sativa ,Citrus sinensis ,Populus alba and Solanurn lycopersicum （82% ,81%,79% and 78%）. Amino acid alignment indicated MaGAD1 has a CaM binding domain at the carboxy terminus and a putative PLP-binding motif. RT-PCR analysis showed that MaGAD1 is expressed in roots, stems, leaves, flowers and fruits. The expression lever is higher in fruits than in other organs. Real time quantitative RT-PCR analysis and measure of ethylene production indicated that the expression levels of MaGAD1 are induced by ethylene production from fruit and improve ethylene biosynthesis in banana fruit natural ripeness.
出处
《西北植物学报》
CAS
CSCD
北大核心
2009年第3期429-434,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
热带生物技术研究所中央级公益性科研院所基本科研业务专项(ITBBZD0733)
公益性行业(农业)科研专项(nyhyzx07-029)
关键词
香蕉
谷氨酸脱羧酶
克隆
表达分析
实时定量PCR
banana
glutamate decarboxylase
cloning
expression analysis
real-time quantitative PCR
