摘要
背景:从组织工程心脏瓣膜材料的来源、瓣膜脱细胞方法、组织工程瓣膜的保存、组织工程心脏瓣膜的钙化、生物反应器及细胞与材料的黏附几方面进行归纳总结,为组织工程心脏瓣膜的临床应用提供理论依据。方法:应用计算机检索检索中国期刊全文数据库、万方数据库2000-01/2008-12期间的相关文章,检索词"瓣膜;心脏;组织工程",限定文章语言种类为中文。共检索到50篇文献,对资料进行初审,纳入标准:与心脏瓣膜支架材料相关的文章。排除标准:重复性研究。结果:目前临床治疗心脏瓣膜病常用的瓣膜替代物主要是机械瓣和生物瓣。所使用的瓣膜支架材料大多属于生物"惰性"材料,不能为种子细胞的附着和生长提供良好的生物界面。研究表明,选取低渗液一SDS和核酸酶共同对猪主动脉瓣膜进行脱细胞处理效果较好。动物实验证明环氧氯丙烷能使戊二醛处理的瓣膜钙化明显减轻,且瓣膜的力学性能和组织稳定性良好。有研究发现,将促黏附分子RGD肽(精氨酸一甘氨酸一天冬氨酸)对胶原材料表面修饰,可提高生物源性支架的细胞黏附性,促进组织工程心脏瓣膜构建。结论:异种生物源性脱细胞的生物瓣膜是常用的生物材料之一其在免疫原性方面基本满足了作为组织工程心脏瓣膜支架材料要求,但其他方面的特性如机械力学性能、细胞亲合性等还需进一步验证。
OBJECTIVE： This study summarized source of tissue-engineered cardiac valve materials, acellular method, storage and calcification of tissue-engineered cardiac valve, bioreactor, and adhesion of cell to materials in order to provide evidences for clinical application of tissue-engineered cardiac valve. METHODS： A computer-based online search was conducted in CNKI and Wanfang database for Chinese language publications containing the key words of ＂valve, heart, tissue engineering＂ from January 2000 to December 2008. There were 50 literatures in total. Articles related to cardiac valve scaffold were included, but duplicated articles were excluded. RESULTS： Mechanical prosthetic valve and bioprosthetic valve have been recently reported to treat valvular disease of the heart in clinic. However, a lot of scaffold materials are inertia, so they are not to provide a great biological interface for cell adhesion. Research indicated that hypotonic solution （SDS and nuclease） has great effects on acellular processing of porcine aortic valve. Animal experiments demonstrated that epichlorohydrin could relieve glutaraldehyde-induced valvular calcification, and mechanical property and stability of valve were well. Some additional studies reported that collagen materials modified by arginine-glycine-aspartic acid could enhance cell adhesion of biological scaffolds and promote construction of tissue-engineered cardiac valve. CONCLUSION： Heterogenous biology-derived acellular biovalve is frequently used due to its immunogenicity beneficial for being tissue-engineered cardiac valve materials; however, its mechanical property and hydrophilicity need to be further studied.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第12期2333-2336,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
