摘要
背景:课题组在前期研究中发现银屑病患者骨髓造血细胞活性存在异常。目的:观察银屑病患者骨髓基质细胞分泌干细胞因子和粒细胞集落刺激因子的水平。设计、时间及地点:病例-对照观察,于2007-10/2008-08在太原市中心医院皮肤科实验室完成。对象:选择太原市中心医院皮肤科临床及病理确诊为寻常型银屑病的门诊患者24例,男16例,女8例;年龄16～59岁;另取血液科骨穿后经筛选的正常骨髓20例作为对照组,性别、年龄与银屑病患者匹配。方法:采用密度梯度离心法分离银屑病患者与对照组骨髓单一核细胞,通过贴壁法培养骨髓基质细胞,收集传了3代后又培养72h的骨髓基质细胞及培养上清。主要观察指标:流式细胞仪检测细胞表面标志并用ELISA法检测上清液中干细胞因子和粒细胞集落刺激因子的水平。结果:流式细胞仪检测结果显示,90%以上细胞表面抗原高表达CD29,而CD34、CD45及HLA-DR表达阴性,即骨髓基质细胞纯度在90%以上。银屑病组骨髓基质细胞培养上清液的干细胞因子和粒细胞集落刺激因子表达均显著高于对照组(P<0.01)。结论:银屑病患者骨髓基质细胞分泌干细胞因子和粒细胞集落刺激因子存在异常,提示银屑病患者骨髓造血微环境发生了改变。
BACKGROUND： It is discovered in our preliminary study that the activity of psoriatic bone marrow hematopoietic cells is abnormal. OBJECTIVE： To reveal level of stem cell factor （SCF） and granulocyte colony-stimulating factor （G-CSF） secreted by bone marrow stromal cells in psoriasis patients. DESIGN, TIME AND SETTING： This case-control observation experiment was performed at the Laboratory of Department of Dermatology, Taiyuan Central Hospital from October 2007 to August 2008. PARTICIPANTS： Twenty-four common-psoriasis outpatients were selected at the Department of Dermatology, Taiyuan Central Hospital, including 16 males and 8 females, aged 16-59 years old. Twenty controls were selected from Department of Hematology, Taiyuan Central Hospital, whose age and gender were matched with psoriasis patients. METHODS： Bone marrow mononuclear cells were isolated from psoriasis patients and controls using the density gradient centrifugation. Bone marrow stromal cells were cultured by the adherent method. When the cells were cultured for three passages and 72 hours, bone marrow stromal cells and supernatant liquid were collected. MAIN OUTCOME MEASURES： The phenotypes of ceils were identified by flow cytometry and the level of SCF and G-CSF were detected by enzyme linked immunosorbent assay kit （ELISA）. RESULTS： Flow cytometry assay showed that over 90% bone marrow stromal cells were positive for CD29, but negative for CD34, CD45 and HLA-DR. That is, the purity of bone marrow stromal cells was over 90%. The levels of SCF and G-CSF in psoriasis patients were significantly higher than that in controls （P 〈 0.01 ）. CONCLUSION： SCF and G-CSF are abnormal which secreted by psoriatic bone marrow stromal cells. This shows that psoriatic bone marrow microenvironment may be abnormal.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第14期2713-2716,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(30771940)~~
