摘要
【目的】华重楼内生菌PCE45具有较强的抗菌活性,本文将对PCE45产生的抗菌物质进行分离纯化和性质分析报道。【方法】PCE45发酵液经硫酸铵盐析、丙酮沉淀、SephadexG75柱、DE52纤维素柱和SephadexG25凝胶柱纯化分离得到抗菌肽PCP-1。【结果】稳定性测试表明该抗菌肽对蛋白酶不敏感,对高温、强酸、强碱有较好的耐受性,可造成稻瘟病菌菌丝畸形并抑制孢子萌发。抑菌谱表明该抗菌肽对玉米弯孢病菌等真菌和大肠杆菌等细菌有较强的抑菌效果。质谱测得其分子量为1058.3。氨基酸组成分析表明该小肽主要由7种氨基酸组成。茚三铜反应呈阴性,酸水解后,茚三酮反应和双缩尿反应呈阳性。【结论】根据茚三酮反应和双缩尿反应结果推测PCP-1可能为低分子量的环状小肽。这是首次关于华重楼内生菌抗菌肽的研究报道。
[ Objective] We isolated an endophyte PCE45 from the rhizome of Pads polyphylla var. chinensis. From PCE45, we purified and characterized an antimicrobial peptide. [ Methods] After ammonium sulfate salting-out, acetone precipitation, SephadexG75,DE52 and SephadexG25 column chromatography, we separated an antimicrobial peptide PCP-1 from the strain PCE45. The stability against high temperature and proteinase, and antimicrobial activity were also analyzed. [ Results] The antimicrobial peptide PCP-1 was stable to proteinase and tolerated high temperature, strong acid and strong base. PCP-1 caused deformation of the hyphae of Pyricularia oryzae and prohibited the spore germination. It also inhibited fungi such as Curvularia lunata and bacteria such as Escherichia coli. Mass spectrogram measurement revealed its molecular weight of 1058.3 Da. The amino acid composition of the peptide composed of 7 amino acids. Ninhydrin reaction showed negative trait whereas after acid hydrolysis with positive ninhydrin reaction and biuret reaction. [ Conclusion] The ninhydrin reaction and biuret reaction imply that the peptide PCP-1 is a cyclic lipeptide. This is the first report about antimicrobial peptide from Paris polyphylla var. chinensis .
出处
《微生物学报》
CAS
CSCD
北大核心
2009年第4期498-503,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金(30670218)~~
关键词
华重楼
内生菌
抗菌肽
纯化
Paris polyphylla var. chinensis
endophyte
antimicrobial peptide
purification
