摘要
为建立简单、快速的西瓜诱导不定芽分化的试验体系，以西瓜‘京欣一号’父本为材料诱导无菌苗，接种4～5d苗龄的子叶和下胚轴进行组织培养，结果表明：采用子叶为外植体诱导的不定芽数量比下胚轴多；以MS培养基为基本培养基，添加BA1．0mg／L和IAA0．5mg／L时为最佳不定芽诱导培养基，添加0．1mg／L KT为最适合的不定芽伸长培养基，添加NAA0．2mg／L的1／2Ms培养基为最佳生根培养基。
Cotyledons and hypocotyls at 4 - day parent were used to study regeneration system seedlings induced by seeds of ‘Jing Xin No. 1＇ male The results showed that： cotyledons produced much more shoots than hypocotyls; MS supplemented with BA 1.0 mg/L and IAA 0.5 mg/L was the best shoot regeneration medium; To shoot growth, the MS supplemented with 0.1 mg/L KT medium was better than others; 1/2 MS supplemented with NAA 0.2 mg/L was the best rooting medium.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2009年第2期58-60,65,共4页
Journal of Hebei Agricultural University
基金
河北省自然科学基金资助项目(303188
C2007000547)
关键词
西瓜
子叶
下胚轴
组织培养
watermelon
cotyledon
hypocotyl
tissue culture
