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Sp1在人肺癌细胞中对survivin表达的调控 被引量：2

Regulation of Survivin Expression by Transcription Factor Sp1 in Human Lung Cancer Cell
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摘要 [目的]探讨Sp1转录因子在人肺腺癌细胞株A549中对survivin基因表达影响。[方法]电泳迁移率分析(EMSA)检测A549细胞核蛋白与标记的survivin启动子序列在有、无mithramycin时的结合情况。用不同浓度mithramycin处理A549细胞,RT-PCR检测survivin mRNA的表达。[结果]标记的survivin启动子序列-126bp～-106bp与核蛋白作用,出现DNA-核蛋白结合条带,经mithramycin预处理的相同序列出现减弱的DNA-核蛋白结合条带;序列-186bp～-166bp与核蛋白作用后无相应条带。mithramycin使A549细胞survivin mRNA表达显著下降。[结论]survivin启动子-121bp～-116bp处存在Sp1结合位点,mithramycin抑制Sp1与此位点的结合;-178bp～-175bp处可能不存在Sp1结合位点。mithramycin抑制survivin mRNA表达,抑制作用具有浓度依赖性。
作者 张晓洁 陈余清 李伟
机构地区 蚌埠医学院第一附属医院 杭州市红十字会医院
出处 《中国肿瘤》 CAS 2009年第4期319-321,共3页 China Cancer
基金 国家自然科学基金资助(30772532)
关键词 肺肿瘤 Sp1转录因子 SURVIVIN MITHRAMYCIN A549细胞
分类号 R734.2 [医药卫生—肿瘤]
  • 相关文献

参考文献6

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二级参考文献19

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共引文献39

同被引文献23

  • 1陈余清,李殿明,蔡映云,刘超,夏雪梅,胡俊锋.纤维支气管镜活检病变组织标本和痰标本Survivin基因的检测对肺癌的诊断价值[J].中华结核和呼吸杂志,2005,28(4):225-229. 被引量：40
  • 2Wang L,Wei D,Huang S,et al.Transcription factor Sp1 is a significant predictor of survival in human gastric cancer[J].Clin Cancer Res,2003,9(17):6371-6380.
  • 3Jiang NY,Woda BA,Banner BF,et al.Spl,a new biomarker that identifies a subset of aggressive pancreatic ductal adenocarcinoma[J].Cancer Epidemiol Biomarkers Prev,2008,17:1648-1652.
  • 4Kuo L,Chang HC,Leu TH,et al.Src oncogene activates MMP-2 expression via the ERK/Sp1 pathway[J].J Cell Physiol,2006,207:729-734.
  • 5Safe S,Abdelrahim M.Sp transcription factor family and its role in cancer[J].Eur J Cancer,2005,41:2438-2448.
  • 6Tapias A,Ciudad CJ,Roninson IB.et al.Regulation of Spl by cell cycle related proteins[J].Cell Cycle,2008,7(18):2856-2867.
  • 7Lou Z,O'reillk S,Liang H,et al.Down-regulation of overexpressed Sp1 protein in human fibrosarcoma cell lines inhibits tumor formation[J].Cancer Res,2005,65 (3):1007-1017.
  • 8潘奇,王鲁,孙惠川,李涛,张巨波,钱永兵,周俭,樊嘉,汤钊猷.转录因子Sp1在肝癌细胞VEGF表达调控中的作用[J].中国临床医学,2007,14(4):487-490. 被引量：15
  • 9CSIKI I,YANAGISAWA K,HARUKI N.Thioredoxin-1modulates transcription of cyclooxygenase-2 via hypoxia-inducible factor-1 in non-small cell lung cancer. Cancer Research . 2006
  • 10CHEN Y Q,ZHAO C L,LI W.Effect of hypoxia-induciblefactor-1αon transcription of survivin in non-small cell lungcancer. Journal of Experimental and Clinical Cancer Research . 2009

引证文献2

二级引证文献4

中国肿瘤
2009年 第4期

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