摘要
猪繁殖与呼吸综合征(PRRS)是引起猪繁殖障碍的主要疫病之一。目前对PRRSV的致病机制和自身复制机制尚不完全明确,因此不能从根本上清除PRRSV的存在。这就使得对PRRSV的检测显得尤为重要。国内外研究表明用于猪繁殖与呼吸综合征检测的方法主要有酶联免疫吸附试(ELISA)、间接荧光抗体试验(IFA)、病毒分离、RT-PCR,同时一些新的检测方法如套式PCR,双重实时荧光定量PCR以及RT-LAMP技术也相继建立起来。
Porcine reproductive and respiratory syndrome(PRRS),characterized by reproductive failure and respiratory disease in pigs is a major disease in the swine industry worldwide.Currently,its pathogenic mechanisms and self-replication mechanism are not entirely clear,so the detection of PRRSV is particularly significant.Various assays have been developed for detection of PRRSV,for example,enzyme-linked immunosorbent assay(ELISA),indirect fluorescent assay(IFA),virus isolation and RT-PCR.Meanwhile,some new detection methods,such as nested-PCR,duplexreal-time RT-PCR,as well as RT-LAMP have been set up.
出处
《动物医学进展》
CSCD
北大核心
2010年第S1期202-205,共4页
Progress In Veterinary Medicine
