摘要
利用液体选择培养基、固体选择培养基从污泥中筛选出一种可以氧化低价硫的菌株,该菌为革兰氏阴性菌,在光学显微镜下有较强的运动能力.对含有该菌的培养基进行pH值检测,在培养的第3天发现pH值有显著下降,继续培养一周左右,pH值下降到0.6;用离子色谱进行检测,培养5天,液体培养基中硫酸根含量从249.2mg.L-1增加到3679.7mg.L-1;将菌液加入FeS培养基中,培养2周后,FeS被氧化,培养基颜色变浅.
A sulfur-oxidizing bacterial strain was isolated from sewage sludge using liquid selective medium and then purified on solid selective medium.The bacteriam can metabolize sofur or S2O2-3to SO2-4 during growth.The sulfur-oxidizing strain is Gram-negative,and has ability to swim.After three days of cultivation,the results showed that the pH of medium decreased.After one week,the pH of the medium dropped to about 0.6,which suggests sulfur oxidizion is taken place.The concentration of the sulfate was tested by using ion chromatography,and the results showed that the concentration of the sulfate increased from 249.2 mg·L-1 to 3 679.7 mg·L-1.The sulfur-oxidizing bacterium was then inoculated into the FeS medium,as expected,the FeS was oxidized and the media became lighter in color.
出处
《北京科技大学学报》
EI
CAS
CSCD
北大核心
2007年第S2期212-215,共4页
Journal of University of Science and Technology Beijing
基金
北京市教育委员会共建项目建设计划
关键词
硫氧化菌
菌种筛选
表征
sulfur-oxidizing bacterium
isolation
characterization
