摘要
旨在观察阿司匹林对脂多糖和地塞米松分别诱导的小鼠腹腔巨噬细胞凋亡中caspase-3表达的影响.小鼠腹腔巨噬细胞培养成功后,分别以脂多糖(LPS)和地塞米松(Dex)为诱导剂进行凋亡的诱导,在出现凋亡的基础上,给予不同剂量的阿司匹林分别分5组给药,检测caspase-3表达情况.mRNA水平采用RT-PCR方法,荧光定量法分析酶活性.结果显示阿司匹林对脂多糖诱导的凋亡有作用。对地塞米松诱导的凋亡无明显作用.
The order of the experi is to evaluate the function of Asprin during the apoptosis of macrophages induced by lipopolysaccharide(LPS) and Dexamechesone(Dex). After the success of culture of the mices' macrophages, give them IPS,Dex to induced apoptosis. On the base of apoptosis, giving cells the Asprin respectively. After that, RT-PCR and Fluorescent anylysis are used to determine the expression of caspase-3. The result is Asprin has the acceleration on caspase-3 induced by LPS, has no special effects on caspase - 3 induced by Dex.
出处
《云南大学学报(自然科学版)》
CAS
CSCD
2004年第S2期9-12,共4页
Journal of Yunnan University(Natural Sciences Edition)
基金
贵州省科技厅资助项目