摘要
代谢工程解决的主要问题就是改变某些途径中的碳架物质流量或改变碳架物质流在不同途径中的流量分布,其目标就是修饰初级代谢,将碳架物质流导入目的产物的载流途径,以获得产物的最大转化率.作者通过对途径分析方法确定了枯草芽孢杆菌生产鸟苷的最佳途径的代谢通量分布,通过对所确定的枯草芽孢杆菌生产鸟苷的3种基础模型进行比较,提出增加鸟苷代谢流的策略--NH4+和PO43-扰动,并进行试验验证.结果表明,扰动后鸟苷的产量增加至23.68g/L.
The primary problem that metabolic engineering should resolve is to restructure the metabolic flux in some pathways or to redirect the flux distributions in different pathways. The target is to trim the primary metabolism, to introduce the metabolic flux into the flux of end product. Pathway analysis for production of guanosine by Bacillus subtillis was conducted in this paper. Theoretical yield and flux distribution for optimal pathways were determined. Based on the comparision among three basic modes determined, the strategies of interaction with NH4+ and PO43- were indicated which provide theory guide for the fermentation process. The production of gunosine was increased to 23.68 g/L after interaction.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
2004年第S2期74-78,共5页
Journal of Yunnan University(Natural Sciences Edition)
基金
天津市高等学校科技发展基金项目(20031205)
关键词
鸟苷
途径分析
条件优化
L-guanosine
pathway analysis
optimization condition
