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Increment of antioxidase activity of transgenic tobacco with betaine aldehyde dehydrogenase 被引量:4

Increment of antioxidase activity of transgenic tobacco with betaine aldehyde dehydrogenase
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摘要 Superoxide dismutase (SOD) activity in the leaves of transgenic tobacco plants with betaine aldehyde dehydrogenase (BADH) gene was about 36% higher than that in the control plants (parent plants), activities of per-oxidase (POD) and catalase (Cat) increased by about 62% and 88% respectively. Activities of ascorbate peroxidase (AsSPOD), dehydroascorbate redutase (DAsAR) and glu-tathione reductase (GR) in ascorbate-glutothion pathway located at chloroplasts increased by 67.7%, 47.9% and 38.8% respectively. These results indicated that the H2O2 produced by SOD catalyzing superoxide anion radicals (O2-) could be fully decomposed, and could not derive to form the strongest toxicant radicals · OH. This is the first report to elucidate quantitatively that the activities of two kinds of antioxidative enzymes decomposed radicals and active oxygen were matched. Photoinhibition tolerant capacity of the transgenic tobacco plants was 35% higher than that in the parent plants. Increment of photoinhibition tolerant Superoxide dismutase (SOD) activity in the leaves of transgenic tobacco plants with betaine aldehyde dehydrogenase (BADH) gene was about 36% higher than that in the control plants (parent plants), activities of peroxidase (POD) and catalase (Cat) increased by about 62% and 88% respectively. Activities of ascorbate peroxidase (AsSPOD), dehydroascorbate redutase (DAsAR) and glutathione reductase (GR) in ascorbate-glutothion pathway located at chloroplasts increased by 67.7%, 47.9% and 38.8% respectively. These results indicated that the H2O2 produced by SOD catalyzing superoxide anion radicals (O2 ?) could be fully decomposed, and could not derive to form the strongest toxicant radicals · OH. This is the first report to elucidate quantitatively that the activities of two kinds of antioxidative enzymes decomposed radicals and active oxygen were matched. Photoinhibition tolerant capacity of the transgenic tobacco plants was 35% higher than that in the parent plants. Increment of photoinhibition tolerant capacity in the transgenic tobacco plants might be due to increment of antioxidative enzymes activities, in turn being able to more effectively scavenge active oxygen and radicals, protect organization and function of chloroplasts. These results showed that the increment of antioxidative enzymes activities in the transgenic tobacco might be one of the reasons for the increment of resistance in the transgenic tobacco.
出处 《Chinese Science Bulletin》 SCIE EI CAS 2001年第6期492-495,共4页
基金 the Stale Key BasicResearch Developmental Program of China (Grant No. (11999011700) and the Natural Science Foundation of Shandong (Gram No. Y9X02037).
关键词 betaind ALDEHYDE DEHYDROGENASE gene TOBACCO antioxi- dative ENZYMES activities. betaind aldehyde dehydrogenase gene tobacco antioxidative enzymes activities
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