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Development of a quantitative RT-PCR system for the specific detection of human heat shock mRNA expression

Development of a quantitative RT-PCR system for the specific detection of human heat shock mRNA expression
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摘要 Heat shock proteins (HSPs), as molecular chaperones, play an important role under physiological condition and in the course of many diseases. It would therefore be valuable to determine the expression of cellular hsp gene quantitatively. Using DNA recombinant technique and in vitro transcription system, a complex internal control RNA has been prepared. After opti- Heat shock proteins (HSPs), as molecular chaperones, play an important role under physiological condition and in the course of many diseases. It would therefore be valuable to determine the expression of cellularhsp gene quantitatively. Using DNA recombinant technique andin vitro transcription system, a complex internal control RNA has been prepared. After optimization of conditions a RT-PCR system for specific quantification of cellularhsp70, hsp90α andhsp90β mRNA has been developed for the first time.
机构地区 Chinese Acad Med Sci
出处 《Chinese Science Bulletin》 SCIE EI CAS 1999年第22期2058-2062,共5页
关键词 HEAT shock GENE MRNA QUANTITATIVE RT-PCR. heat shock gene mRNA quantitative RT-PCR
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参考文献11

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二级参考文献1

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