摘要
目的 了解山东地区输血后丙型肝炎和非甲～庚型肝炎病例中TTV感染及基因变异的状况。方法应用套式PCR方法检测41例输血后丙型肝炎和16例非甲～庚型肝炎患者血清中TTV DNA,并对其中阳性的2例扩增产物直接克隆测序,分析其基因变异的情况。结果 41例输血后丙型肝炎患者血消中TTVDNA阳性检出率为19.5％(8/41),而16例非甲～庚型肝炎患者血清中阳性检出率为31.3％(5/16)。TTV山东第一株序列和山东第二株序列相应位置核苷酸同源性与日本株和中国第一株相比较分别为94％和91％;山东TTV的两株序列间同源性为91％。结论 本项研究证实山东地区输血后丙型肝炎和非甲～庚型肝炎患者中存在较高TTV感染,输血可能为传播TTV感染途径之一,是否该地区存在着不同的TTV亚型还有待于进一步调查证实。
Objective To investigate the prevalence of TTV infection and genetic variation of TTV inShandong Province. Methods TTV DNA were amplified and detected by PCR from sera of patients with post-tran-fusion hepatitis C or with Non A-G hepatitis. The PCR products were sequenced and analyzed. Results TTV DNA were positive in 8 of 41 patients with posttransfusion hepatitis C(19. 5%), and in 5 of 16 with Non A-G hepatitis (31.3%). The homology comparison of TTV DNA sequences showed that our first TTV isolate were both 94% to AB008394 in Japan or CHIN1 in China,and the second isolate were 91% and 93% .respectively. Conclusions High TTV infection rate exists in Shondong and blood transfusion may be one of TTV transmission routes.
出处
《实用肝脏病杂志》
CAS
1999年第3期145-147,共3页
Journal of Practical Hepatology
关键词
TTV
输血后丙型肝炎
非甲~庚型肝炎
聚合酶链反应
核苷酸序列
Transfusion Transmitted Virus Post-transfusion Hepatits C Non A-G Hepatitis Polymerase Chain Reaction Nucleotide Sequence.
