摘要
目的:探讨肝络通降低胆汁性肝纤维化引起的大鼠门静脉高压的作用机制。方法:取体质量240-260g左右的清洁级SD雄性大鼠156只,根据体质量随机分为假手术组、模型组、心得安组、肝络通高、中、低剂量组。采用胆总管结扎的方法造成大鼠胆汁性肝纤维化模型。术后2周和4周分别测定各组的门静脉压力,HE染色观察肝组织学的变化,检测血清肝功能,放免法定量体循环和肠系膜上动脉血浆内皮素-1的浓度。结果:术后2周和4周,模型组大鼠门静脉压力显著升高。心得安和肝络通在两个时间点有显著的降压作用。肝络通能显著抑制胶原生成,促进肝组织结构的恢复;肝功能显示肝络通在两个时间点均能显著降低血清丙氨酸氨基转移酶(AST)、门冬氨酸氨基转移酶的活性(ALT),血清总蛋白(TP)、白蛋白(ALB)、总胆红素(TBIL)的含量和Na+的浓度。门静脉高压症初期肝络通降低体循环内皮素-1的浓度,提高肠系膜循环的内皮素-1的浓度,抑制内脏血管的舒张。结论:肝络通通过改善肝功能,抑制胶原生成,调节肝内微循环,改善纤维化初期高动力循环,抑制水钠潴留,从而降低门静脉压力。
Objective： To investigate the mechanism of Glytan lowering portal pressure induced by biliary liver fibrosis. Methods： SD male rats, 240-260g weight around, were randomly divided into sham-operation group, model group, propranolol group, Glytan high-dose, middle-dose and low-dose group according to the weight. Portal hypertension was induced by common bile duct ligation in rats. After two and four weeks, measured the portal pressure（PP） of each group, observed the histological changes of liver by HE staining, tested liver function and the concentration of endothelin-1 in systemic circulation and mesenteric circulation radioimmnuoassay. Results： After two and four weeks, portal pressure of model group rats increased significantly. Both Glytan and propranolol can decrease PP after two and four weeks, and the pressure-relief effect was similar between the two drugs. HE staining showed that Glytan can significantly inhibit the formation of collagen, promote the recovery of liver tissue structure; liver function indicated a significant decrease in serum AST, ALT, TBIL and Na＋ concentration. In addition, Glytan decreased the concentration of endothelin -1 in systemic circulation, increased it in mesenteric circulation after two weeks. Conclusion： Glytan decrease PP by improving liver function and microcirculation, inhibiting collagen formation and water-sodium retention after long-term therapy, ameliorating hyperdynamic circulation at the early stage.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2009年第4期446-451,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
教育部长江学者和创新团队发展计划资助项目(No.IRT0413)
关键词
门静脉高压症
肝内微循环
高动力循环
肝络通
实验研究
Portal hypertension
Liver microcirculation
Hyperdynamic circulation
Glytan
Experimental study
