摘要
在46只清洁级纯种昆明小白鼠上进行了不同水温环境下,70分钟游泳运动对小鼠体温以及脑、肝、腓肠肌和心肌组织MDA含量,超氧化物歧化酶(SOD)活性水平、总抗氧化能力及肌糖元和肝糖元含量的影响的研究.结果如下:1.安静组维持在较高水平,p<0.05(数值分别为:肝419.80±47.56NU/ml上升到483.18±38.55NU/ml;脑149.3±38.87NU/ml上升到181.25±9.74NU/ml;腓肠肌101.37±7.20NU/ml上升到122.05±10.97NU/ml;心肌325.52±48.16NU/ml上升到381.63±37.97NU/ml),同时脑的MDA含量较安静组保持在较低水平,由安静组的6.01±2.26nm/ml下降为游泳组的4.59±0.65nm/ml(P<O.05),在该温度下,肝糖元和肌糖元都能够较充分地被利用,利于超量恢复.2.在33℃水温条件下的70分钟游泳对机体刺激较小.游泳组MDA,SOD和总抗氧化能力较安静组1在本实验所设备的温度中,28℃水温条件下,游泳组四种组织SOD抗氧化酶活性较均无显著性变化.3.38℃水温条件下运动对机体有较大损伤.70分钟游泳后体温高于安静组(游泳组为35.6±1.8℃,安静组为33.9±1.2℃),游泳组肝脏和心肌的MDA含量均显著上升,P<0.05(肝由11.59±3.27nm/ml上升到16.29±2.69nm/ml,心肌由13.03±1.18nm/ml上升到16.86±2.61nm/ml);在该温度下,肝糖元和肌糖元的消耗受抑制。
The experiment was performed in 46 Kunming mice. The influences of three different water temperatures (28℃,33℃ and 38℃)on core temperature, malonaldehyde (MDA)content, superoxide dismutase (SOD) activity level, total antioxidize capacity(TAC)in four tissues (brain, liver, musculus gastrocnemius and cardiomuscle), hepatic glycogen concentration and muscle glycogen concentration response to 70 min swimming were studied. Three groups of mice after swimming were observed in com-parision with resting groups under different water temperature conditions. The results are as follows:1. At controlled water temperature (28℃), SOD activity of swimming group in four tissues (brain, cardiomuscle, liver and musculus gastrocnemius) remained at a higher level, p<0. 05(brain 181. 25± 9. 74NU/ml vs 149. 3±38. 87NU/ml; cardiomuscle 381. 63±37-97NU/ml vs 325. 52±48. 16NU/ml; liver 483.18±38. 55NU/ml vs 419. 80±47. 56NU/ml( musculus gastrocneius 122. 05±10. 97NU/ml vs 101. 37±7. 20NU/ml), than that of the resting group. MDA content of swimming group in brain remained at a lower level, p<0. 05(4.59±0. 65nm/ml vs 6. 01±2. 26nm/ml), than that of the resting group. At this temperature (28℃), hepatic glycogen and muscle glycogen can be used fully by the swimming group, which is beneficial to over-recovery.2. at 33℃ water temperature, mice bodies were stimulated moderately by swimming exercise. MDA, SOD and TAC indicate no remarkable difference between the swimming group and the resting group.3. At 38℃ water temperature, mice bodies were injured remmarkably by 70 min swimming exercise. After exercise, mice body temperatures of the swimming group were higher than those of the resting groups (35.6±1.8℃vs 33. 9±1.2℃); MDA content in liver and cardiomuscle increases signifi-cently in comparision with the resting group (liver 16. 29±2. 69nm/ml vs 11. 59±3. 27nm/ml; cardiomuscle 16. 86±2. 61nm/ml' vs 13. 03±1. 18nm/ml). In addition, at this temperature, the consumption of hepatic glycogen and muscle glycogen is restrained at a high level.4. Comparing SOD activity in four tissues at three different water temperatures, we find that, within certain range, an increase of SOD activity accompanied by the increase of difference between body temperature and environment temperature is beneficial to remove excessive free radicals in body.5. Comparing the range of SOD activity in four tissues, we find that, SOD activity sequence is : musculus gastrocnemius<brain<cardiomuscle<liver. TAC in four tissues has the same sequence: musculus gastrocnemius<brain<cardiomuscle<liver.6. By covariance analysis, the interaction of water temperture factor and swimming factor apparently exits.The results indicate: swimming exercise at 28℃ water temperature has beneficial influence on body protection and improvement of swimming performance. At high water temperature, long time exercise will lead to an increase of body temperature and lipid peroxidation level. So, in exercises and training at high temperature, protection of injury caused by free radicals in body has important role in prevention of fatigue during exercise.
出处
《南京体育学院学报（社会科学版）》
1998年第1期1-18,共18页
Journal of Nanjing Institute of Physical Education
关键词
水温
体温
丙二醛
超氧化物歧化酶
肌糖元
肝糖元
总抗氧化能力
脂质过氧化
小鼠
water temperature
core temperature
malonaldehyde (MDA)superoxide
dismutase (SOD)
hepatic glycogen
muscle glycogen
total antioxidize
capacity(TAC)
lipid peroxidation level (LPO)
mice
