摘要
Abstract Objective To study the molecular basis of bone marrow stromal cell supporting hematopoiesis. Methods Immunoelectron micrscopic ocalization of adherent protein related to regulation of hematopoiesis was performed by immunocolloidal gold labelling technique in the adhernt layer of longterm cultured human hone marrow stromal cells (LTCHBMSC). Results Adherent proteins were found to be expressed on LTCHBMSC. Double labelling revealed co expression of these proteins and some specific markers for endothelial cells (vWF) and fibroblasts (Fb sp). It was suggested that endothelial cells and fibroblasts in LTCHBMSA could synthesize and secrete adherent proteins, which bound to the surface of stromal cells and extracellular matrix. Conclusion Adherent proteins and all kinds of hematopoietic growth factors secreted by stromal cells constitute a complex network supporting hematopoiesis. Study on the coagulant activity of human blood monocytes. Jia Xiaomei, Zheng Huan, Wang Jing, et al. Institute of Hematology, Beijing People's Hospital, Beijing Medical University, Beijing 100044, China. Chin J Hematol 1997; 18(9) 474 476. Objective To investigate the coagulant and fibrinolytic activities of human blood monocytes. Methods Cultured monocytes (2.8×10 6 cells) were refrigerated at 20℃ and then thawed and centrifuged. Blood coagulant factors activities in the upper and lower layer of the supernatant were assayed and compared with that in the supernatant of white blood cells (containing 2.8×10 6 cells). Results There were factors Ⅱ, Ⅶ, Ⅸ, Ⅹ, Ⅴ, and tissue factor activities in the supernatant, Ⅹ Ⅲ A: Ag was 2.1%, Ⅹ Ⅲ S: Ag: 2.8%, while t PA: A and PAI: A were undetectable. In the lower layer there was no specific expression of blood coagulant factors. Conclusion There were coagulant activities in the supernatant containing the membrane and cytoplasma of monocytes.
Abstract Objective To study the molecular basis of bone marrow stromal cell supporting hematopoiesis. Methods Immunoelectron micrscopic ocalization of adherent protein related to regulation of hematopoiesis was performed by immunocolloidal gold labelling technique in the adhernt layer of longterm cultured human hone marrow stromal cells (LTCHBMSC). Results Adherent proteins were found to be expressed on LTCHBMSC. Double labelling revealed co expression of these proteins and some specific markers for endothelial cells (vWF) and fibroblasts (Fb sp). It was suggested that endothelial cells and fibroblasts in LTCHBMSA could synthesize and secrete adherent proteins, which bound to the surface of stromal cells and extracellular matrix. Conclusion Adherent proteins and all kinds of hematopoietic growth factors secreted by stromal cells constitute a complex network supporting hematopoiesis. Study on the coagulant activity of human blood monocytes. Jia Xiaomei, Zheng Huan, Wang Jing, et al. Institute of Hematology, Beijing People's Hospital, Beijing Medical University, Beijing 100044, China. Chin J Hematol 1997; 18(9) 474 476. Objective To investigate the coagulant and fibrinolytic activities of human blood monocytes. Methods Cultured monocytes (2.8×10 6 cells) were refrigerated at 20℃ and then thawed and centrifuged. Blood coagulant factors activities in the upper and lower layer of the supernatant were assayed and compared with that in the supernatant of white blood cells (containing 2.8×10 6 cells). Results There were factors Ⅱ, Ⅶ, Ⅸ, Ⅹ, Ⅴ, and tissue factor activities in the supernatant, Ⅹ Ⅲ A: Ag was 2.1%, Ⅹ Ⅲ S: Ag: 2.8%, while t PA: A and PAI: A were undetectable. In the lower layer there was no specific expression of blood coagulant factors. Conclusion There were coagulant activities in the supernatant containing the membrane and cytoplasma of monocytes.
