摘要
Objective:To investigate the role of SMB on the growth , differentiation and metabolism of osteoblastlike cells in vitro, we studied the effects of SMB on DNA synthesis and alkaline phosphatase(ALPase) activity of the cloned osteoblast like cells-MC3T3-E1. Methods: The cells were cultured in α-MEM with 0. 3% of fetal bovine serum and treated with SMB at the concentration of 0. 1 - 10. 0 g/L. Results : There was no significant difference in DNA synthesis between the groups in different concentration of SMB and the group of control. But SMB increased ALPase activity in a concentration-dependent fashion in later stage of cells , and up to maximum at the level of 5. 0 g/L , in which concentration , ALPase activity was about 135 % greater than that of control. However ,ALPase activity was inhibited in early stage of cells by the addition of SMB. Conclusion : SMB has the stimulating effect on the activity of osteoblast-like cells in vitro, and may play an important role in accelerating the remodeling of bone in vivo as well.
Ｅｆｆｅｃｔｓｏｆｓａｌｖｉａｍｉｌｔｉｏｒｒｈｉｚａｂｕｎｇ（ＳＭＢ）ｏｎｏｓｔｅｏｂｌａｓｔ－ｌｉｋｅｃｅｌｌｌｉｎ（ｃｌｏｎａｌＭＣ３Ｔ３－Ｅ１ｃｅｌｌｓ）￥（丁寅）ＤｉｎｇＹｉｎ；ＳｕｉｃｈＳｏｍａ；Ｔ．ＴａｋａｎｏＹａｍａｍｏｔｏ；Ｓ．Ｍａ...
