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Molecular cloning of a cDNA related to vernalization(verc203) in winter wheat 被引量：1

Molecular cloning of a cDNA related to vernalization(verc203) in winter wheat
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摘要 A cDNA clone related to the vernalization in winter wheat(verc203)was harvested from the en-riched cold-induced cDNA library of 10~4 pfu with differential screening.The insert of verc203 in λ gt10 vector wassubcloned into the sites between BamH Ⅰ and Hind Ⅲ in pUC19 plasmid after being amplified with PCR.the analysis of the Northern blotting with a probe of verc203 indicated that the verc203 has a negative signalfor the control and the devernalized mRNA and a positive signal for the vernalized winter wheat and non-vernalized spring wheat at about 2.6 kb. A cDNA clone related to the vernalization in winter wheat(verc203)was harvested from the en- riched cold-induced cDNA library of 10~4 pfu with differential screening.The insert of verc203 in λ gt10 vector was subcloned into the sites between BamH Ⅰ and Hind Ⅲ in pUC19 plasmid after being amplified with PCR. the analysis of the Northern blotting with a probe of verc203 indicated that the verc203 has a negative signal for the control and the devernalized mRNA and a positive signal for the vernalized winter wheat and non- vernalized spring wheat at about 2.6 kb.
作者 种康 谭克辉 黄华梁 梁厚果
机构地区 Department of Biology Institute of Botany Institutc of Genetics
出处 《Science China Chemistry》 SCIE EI CAS 1995年第7期799-806,共8页 中国科学（化学英文版）
基金 the National Natural Science Foundation of China
关键词 WINTER wheat VERNALIZATION gene molecular CLONING of cDNA. winter wheat vernalization gene molecular cloning of cDNA.
分类号 S512.1 [农业科学—作物学] Q943.2 [生物学—植物学]
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参考文献15

  • 1David B. Collinge,Dawn E. Milligan,J. Maxwell Dow,Graham Scofield,Michael J. Daniels.Gene expression in Brassica campestris showing a hypersensitive response to the incompatible pathogen Xanthomonas campestris pv. vitians[J]. Plant Molecular Biology . 1987 (5)
  • 2Tan,K.H.Specific protein synthesis in process of vernalization. Botanical Research . 1992
  • 3Metzger,J.D,Burn,J.,Dennis,E.S.et al.DNA methylation as the molecular basis for vernalization:a hypothesis. Plant Physiol.(Suppl.) . 1990
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共引文献14

同被引文献10

  • 1种康,谭克辉,黄华粱,梁厚果.冬小麦春化相关基因cDNA(verc203)片段序列的分析[J].植物生理学报（0257-4829),1997,23(1):99-102. 被引量：10
  • 2Levy Y.Y.,Dean C.The transition to flowering. The Plant Cell .
  • 3Macknight R,Bancroft I,Dean C.FCA a gene controlling flowering time in Arabidopsis,encodes a protein containing RNA-binding domains. Cell . 1997
  • 4ChongK,WangLP,TanKH,HuangHL,LiangHG.Molecularcloningandcharacterizationofvernalization_related (ver)genesinwinterwheat. Physiologia Plantarum . 1994
  • 5ChongK,BaoSL,XT,TanKH,LangTB,ZengJZ,HuangHL,XuJ,XuZH,XuZH.Functionalanalysisofthevergeneusingantisensetransgenicwheat. Physiologia_Plantarum . 1998
  • 6Creelman R A,Mullet J E.Oligosaccharins,brassinolides,and jas-monates:Nontraditional regulators of plant growth,development,and gene expression. The Plant Cell . 1997
  • 7K. H. Tan.Mechanism of plant flowering induced with low temperature. Adv. Plant Physiol. Biochem . 1983
  • 8Ausubel F,Brent R,Kingston R E.Short Protocols in Molecular Biology, 3rd ed. Canada: John Wiley & Sons . 1995
  • 9Koorneef, M,Alouso-Blanco, C,Peeters, A. et al.Genetic control of flowering time in Arabidopsis. Annual Review of Plant Physiology . 1998
  • 10Lobler, M,Lee, J,Parthier, B.Two signaling pathways for jasmonate in barley. 5 th International Congress of Plant Molecular Biology . 1997

引证文献1

  • 1YONG Weidong, CHONG Kang , LIANG Tiebing, XU Zhihong , TAN Kehui and ZHU Zhiqing Research Center for Molecular and Developmental Biology, Institute of Botany, Chinese Academy of Sciences , Beijing 100093, China ,Shanghai Institute of Plant Physiology , Chinese Academy of Sciences , Shanghai 200032, China Corresponding authors.Cloning and characterization of vernalization-related gene (ver203F) cDNA 3'end[J].Chinese Science Bulletin,1999,44(14):1289-1294. 被引量：3

二级引证文献3

Science China Chemistry
1995年 第7期

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