摘要
衰老是受遗传控制的生命现象,基因是生物一切原发信息的来源,细胞老化的原发改变一定是基因水平上的变化。据此,对体外有延缓细胞增殖能力下降的中草药提取物F-23和P-15对诱发基因突变和DNA损伤的保护作用进行了观察。通过鼠伤寒沙门氏菌/哺乳动物微粒体酶测变试验、果蝇性联隐性致死突变(SLRL)试验、V_(79)细胞姊妹染色单体交换(SCE)检测和人体外周白细胞程序外DNA合成(UDS)试验等观察。显示F-23和P-15分别对已知诱变剂2-氨基芴(2AF)、甲磺酸甲酯(MMS)和农药溴氰菊酯及电离辐射等诱发的基因突变和DNA损伤有保护作用。对降低基因突变和DNA损伤与细胞老化的关系作了讨论。
The antiaging action of the extracts from chinese traditional medicines F-23 and P-15 that can moderate the degression of cell multiplicative rate are studied from thier protective effect on induced mutagenesis and DNA damage. The expermental results show that F-23 and P-15 can reduce reverse mutation by 2AF in Salmonella, SLRL rate induced by MMS and Delmethrin in Drosophila melanogaster, SCE value induced by60Co r-ray in V79 cells arid UDS induced by Chlormethini Hydrochloridi in human leucocytes. The relation between mutation and DNA damage and cell aging is discussed in this paper.
出处
《中药药理与临床》
CAS
CSCD
1989年第5期15-17,42,共4页
Pharmacology and Clinics of Chinese Materia Medica
